三倍体薄皮甜瓜高效再生体系和遗传转化体系的建立  

ESTABLISHMENT OF HIGH EFFICIENT REGENERATION SYSTEM AND GENETIC TRANSFORMATION OF TRIPLOID THIN-SKINNED MELON

作　　者：盛慧[1] 陈柏杰[1] 金荣荣[1] 曹虹[1] 张金凤 SHENG Hui;CHEN Baijie;JIN Rongrong;CAO Hong;ZHANG Jinfeng(Harbin Academy of Agricultural Sciences, Harbin 150029, China)

机构地区：[1]哈尔滨市农业科学院,哈尔滨150029

出　　处：《内蒙古农业大学学报（自然科学版）》2017年第6期12-17,共6页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：哈尔滨市科技创新人才研究专项(2012RFQYN043)

摘　　要：目的:建立起高效三倍体薄皮甜瓜再生体系和遗传转化体系。方法:以哈研3号薄皮甜瓜为试材,采用组织培养技术,在二倍体甜瓜再生体系的基础上,摸索外植体类型、外源调节剂种类和配比,以及各阶段的培养条件。结果:三倍体薄皮甜瓜再生最佳外植体为带有叶芽的茎段,芽诱导率为94%;芽诱导培养基最佳组合为:MS+6-BA 1.0 mg/L+KT 0.5 mg/L,Agr 1.0%;芽伸长培养基最佳组合为:MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,Agr 1.2%;壮苗培养基为:MS+6-BA 0.2 mg/L+GA30.1 mg/L,Agr 1.2%;生根培养基最佳组合为:1/2MS+NAA 0.5 mg/L,Agr 1.0%;培养条件为:25℃、14h光照/12h黑暗;确定卡那霉素的筛选浓度为2.5mg/L;确定头孢唑啉钠的除菌浓度为500 mg/L。结论:建立三倍体薄皮甜瓜高效再生体系和遗传转化体系,为今后的大量扩繁和转基因研究打下基础。Objective： To establish a high efficient triploid thin-skinned melon regeneration system and a genetic transformation system. Method： Based on the regeneration system of the diploid melon,the explant type,the type and proportion of the exogenous regulator and the culture of each stage were studied by using the tissue culture technique. condition. Result： The best explants of shoot regeneration were shoots with shoots of 94%. The optimum combination of shoot induction medium was MS ＋ 6-BA 1. 0 mg/L ＋KT 0. 5 mg L,Agr 1. 0%. The optimum combination of bud elongation medium was： MS ＋ 6-BA 0. 5 mg/L ＋ NAA 0. 1 mg/L,Agr 1. 2%; strong seedling culture medium： MS ＋ 6-BA 0. 2 mg/L ＋ GA30. 1 mg/L,Agr 1. 2%; The best combination of rooting medium： 1/2 MS ＋ NAA 0. 5 mg/L,Agr 1. 0%; culture conditions： 25 ℃,14 h light/12 h dark; determine kanamycin screening concentration of 2. 5 mg/L; The concentration of cefazolin sodium was 500 mg/L. Conclusion： The high efficient regeneration system and genetic transformation system of triploid thin-skinned melon were established,which laid the foundation for the future research of mass propagation and transgenic.

关 键 词：三倍体薄皮甜瓜 再生体系 扩繁 转基因 

分 类 号：S652.2[农业科学—果树学]