黄芪注射液对甲状腺辐射损伤改善作用的实验研究  

Effect of Astragalus injection on thyroid radiation injury

作　　者：王津京[1] 侯艳微[1] 张世坤 刘然[1] 曾静[1] 李致远 方毅[1] WANG Jin-jing;HOU Yan-wei;ZHANG Shi-kun;LIU Ran;ZENG Jing;LI Zhi-yuan;FANG Yi(No. 307 Hospital of PLA, Beijing 100071, China;Department of Pathology, Anhui Provincial Hospital, Hefei 230001, China;Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850,China)

机构地区：[1]解放军307医院,北京100071 [2]安徽省立医院病理科,合肥230001 [3]军事科学院军事医学研究院军事认知与脑科学研究所,北京100850

出　　处：《军事医学》2017年第12期983-986,共4页Military Medical Sciences

摘　　要：目的探讨黄芪注射液在碘-131(^(131)I)所致甲状腺辐射损伤防护中的作用。方法二级SD雄性大鼠随机分为3组:对照组、^(131)I照射组、黄芪干预^(131)I照射组,分别给予^(131)I照射组及黄芪干预^(131)I照射组大鼠灌胃11.1 MBq^(131)I,黄芪干预^(131)I照射组大鼠同时腹腔注射400 mg/(kg·d)黄芪注射液。于第2、8周用固相放射免疫法测定各组甲状腺激素水平,8周后取大鼠甲状腺组织制作HE染色石蜡切片。应用0、25、50、100、200 MBq/ml的^(131)I照射甲状腺滤泡癌细胞(WRO)24 h,MTT法及流式细胞检测黄芪0.5 g/L干预及非黄芪干预的WRO的增殖及凋亡。结果与正常对照组比较,^(131)I照射组FT3、FT4明显降低(P=0.021,0.017),甲状腺组织滤泡上皮细胞形态不规则,玻璃样变;与^(131)I照射组比较,黄芪注射液干预^(131)I照射组FT3、FT4明显改善(P=0.033,0.045),甲状腺组织玻璃样变程度有所减轻;体外细胞实验显示,与^(131)I照射组相比,黄芪注射液干预^(131)I照射组甲状腺细胞增殖增加,凋亡减少。结论黄芪注射液对^(131)I导致的大鼠甲状腺功能减退、甲状腺细胞损伤有改善作用。Objective To investigate the effect of Astragalus injection on iodine-131 （131 I） induced thyroid radiation injury. Methods Two-stage SD male rats were randomly divided into three groups： control group, 131I irradiation group and Astragalus intervention 131I irradiation group. 131I irradiation group and Astragalus intervention 131I irradiation group were treated with intragastric administration of 11.1MBq 131I, respectively. At the same time, the Astragalus intervention 131I irradiation group was injected intraperitoneally 400 mg/（ kg . d） Astragalus injection liquid. The levels of thyroid hormone were measured by solid-phase radioimmunoassay in the 2nd and 8th weeks of the experiment. The thyroid tissues from rats were HE stained into paraffin sections after 8 weeks. Administration of 0, 25,50,100,200 MBq/ml into 131I irradiation of thyroid follicular carcinoma cells （ WRO ） lasted 24 hours, the proliferation and apoptosis of WRO in Astragalus membranaceus 0.5 g/L intervention and non-Astragalus intervention were detected by MTT assay and flow cytometry. Results Compared with the normal control group, FT3 and FT4 were significantly decreased in the 131I irradiation group （P = 0. 021,0. 017 ）. The morphological changes of the follicular epithelial cells in the thyroid tissue were irregular and the hyaline degeneration was observed. However, compared with 131I irradiation group, FT3 and FT4 were significantly improved by Astragalus injection （P = 0. 033,0.045 ）, and the degree of vitreous degeneration of thyroid tissue was alleviated. Cell experiments in vitro showed that the proliferation of thyroid cells was increased, but apoptosis was reduced. Conclusion Astragalus injection can improve the thyroid function and thyroid injury induced by 131I in rats.

关 键 词：甲状腺 辐射损伤 黄芪 碘 甲状腺激素类 甲状腺功能减退症 

分 类 号：R818[医药卫生—放射医学]