苗药金乌健骨方对类风湿关节炎滑膜细胞IL-17-STAT3信号影响的研究  

Effects of Jinwu Jiangu recipe on IL-17/STAT3 signals in rheumatoid arthritis synoviocytes

作　　者：马武开[1] 李蓉[1] 宁乔怡 黄颖[1] 唐芳[1] 陆道敏[1] 姚血明[1] MA Wu-kai;LI Rong;NING Qiao-yi;HUANG Ying;TANG Fang;LU Dao-min;YAO Xue-ming(Department of Rhettmatology and Immunology, the Second Affiliated Hospital of Guiyang of Traditional Chinese Medicine, Guiyang 550003, Chin)

机构地区：[1]贵阳中医学院第二附属医院风湿免疫科

出　　处：《中国中药杂志》2018年第3期585-590,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81360580);贵州省高层次创新型人才培养计划——“百”层次人才项目(黔科合平台人才[2016]5650);贵州省民族医学特色重点学科项目(黔学位合字ZDXK[2015]7号)

摘　　要：探讨苗药金乌健骨方总提物对类风湿关节炎成纤维滑膜细胞（RASF）IL^-17-STAT3信号的影响。培养原代RASF,分为正常血清组、金乌健骨方低、中、高剂量组（0.06,0.6,6.0 g·L^-1）和雷公藤多苷（0.03 g·L^-1）组,分别予相应浓度的金乌健骨方和雷公藤多苷干预24 h。PCR检测RORα,RORγt,STAT3 mRNA的表达,Western blot法检测IL-17R,p STAT3的蛋白活性。结果显示,与正常血清组比较,RASF中RORα,RORγt,IL-17R和STAT3 mRNA的表达量均有所下降,具有统计学意义（P〈0.01）,与雷公藤多苷组比较,金乌健骨方高、中剂量组均能下调RORα,RORγt,IL-17R和STAT3 mRNA的表达,具有统计学意义（P〈0.05）,金乌健骨方高剂量组作用更明显,具有统计学差异（P〈0.01）。与正常组比较,各给药组IL-17R,p STAT3的蛋白表达水平明显下降（P〈0.01）。与雷公藤多苷组比较,金乌健骨方高剂量组下调p STAT3蛋白表达的作用更强,具有统计学差异（P〈0.01）。由此可见,苗药金乌健骨方总提物能够下调RASF中RORα,RORγt,STAT3 mRNA的表达,抑制IL-17R,p STAT3的蛋白活性。This paper aimed to investigate the effects of Jinwu Jiangu recipe total extract on the IL^-17/STAT3 signals in rheumatoid arthritis synovial fibroblasts（ RASF）. The primary RASFs were cultured by tissue piece method in vitro,and divided into blank control group,Jinwu Jiangu recipe low dose group,Jinwu Jiangu recipe middle dose group,Jinwu Jiangu recipe high dose group,and tripterygium glycosides control group. They were then treated with corresponding serum free medium,different doses of Jinwu Jiangu recipe total extract（ 0. 06,0. 6,6. 0 g·L^-1）,and tripterygium glycosides（ 0. 03 g·L^-1） respectively for 24 hours. The gene expression levels of RORα,RORγt,and STAT3 mRNA were detected by polymerase chain reaction（ PCR）,and the protein activity of IL-17R and p STAT3 were measured by Western blot assay. The results showed that as compared with blank control group,the expression levels of RORα,RORγt,IL-17R and STAT3 mRNA in RASF were significantly declined（ P〈0. 01）. As compared with tripterygium glycosides control group,Jinwu Jiangu recipe total extract middle dose group and high dose group can down-regulate the expression levels of RORα,RORγt,IL-17R and STAT3 mRNA（ P〈0. 05）,and the effect was more obvious in high dose group（ P〈0. 01）. As compared with blank control group,the protein expression levels of IL-17R and p STAT3 in each treatment group were obviously decreased（ P〈0. 01）. As compared with tripterygium glycosides control group,Jinwu Jiangu recipe high dose group had more obvious effect in downregulating the protein expression of p STAT3（ P〈0. 01）. Therefore,Miao medicine Jinwu Jiangu recipe total extract can down-regulate the expressions of RORα,RORγt,and STAT3 mRNA,and inhibit the protein activity of IL-17R and p STAT3 in RASF.

关 键 词：类风湿关节炎 金乌健骨方 苗药 滑膜细胞 IL-17 STAT3 

分 类 号：R29[医药卫生—民族医学]