姜黄素脂质体载药量与体外抗瘤效果的关系  

Relationship Between Drug Loading Capacity and in Vitro Antitumor Effect of Curcumin Nanoliposomes

作　　者：王雪婷 李宁[1] 刘婷 蒋少鸿 邱昌露 王汀[1] Wang Xueting;Li Ning;Liu Ting;Jiang Shaohong;Qiu Changlu;Wang Ting(School of Pharmacy,Anhui Medical University, Hefei,Anhui, China 23003)

机构地区：[1]安徽医科大学药学院,安徽合肥230032

出　　处：《中国药业》2018年第8期1-5,共5页China Pharmaceuticals

基　　金：安徽省高校自然科学研究基金重大项目[KJ2016SD28]

摘　　要：目的探讨不同载药量的姜黄素纳米脂质体(简称CUR-NL)对不同肿瘤细胞的抑制效果。方法采用薄膜分散法制备CUR-NL,以低速离心法测定包封率。噻唑蓝(MTT)法检测不同载药量的CUR-NL对Hep G2细胞、SGC-7901细胞、Bel-7402细胞的抑制作用。结果 CUR-NL(0.5 mg)与CUR-NL(0.25 mg)的包封率分别为(87.06±2.37)%和(95.27±2.60)%;CUR-NL对上述3种肿瘤细胞均有抑制作用,抑制作用呈时间-剂量依赖性;当给药总剂量相同时,载药量与抗瘤效果并不呈正相关。结论对于Hep G2,SGC-7901,Bel-7402肿瘤细胞而言,提高姜黄素载药量并不能提高抗瘤活性。Objective To investigate the relationship between the inhibitor7 effects on tumor cells and drug loading capacity of curcuminnanoliposomes（CUR- NL）. Methods CUR- NL was prepared by thin-film dispersion method and the entrapment efficiency was mea-sured by low-speed centrifugation. The inhibitor7 effects of CUR-NL on HepG2 cells,SGC-7901 cells and Bel-7402 cells wereexplored by MTT assay. Results The entrapment rates of CUR-NL（0.5 nag） and CUR-NL（0.25 nag） were （87.06±2.37）% and（95.27 ±2.60）% ,respectively. CUR-NL had higher inhibitor7 effect on the three tumor cells in the time and dose-dependent manner.When the total dose was identical, CUR- NL with different curcumin loading capacities did not show a difference in the antitumoreffect. Conclusion Enhancement of drug loading capacity of curcumin can＇t enhance in vitro antitumor effects on HepG2,SGC- 7901and Bel- 7402 tumor cells.

关 键 词：药剂学 纳米脂质体 姜黄素 载药量 抗瘤活性 

分 类 号：R285.5[医药卫生—中药学]