高效液相色谱法测定利卡西平血药浓度  

Determination of Blood Concentration of Licarbazepine by HPLC

作　　者：周清武[1] Zhou Qingwu(Department of Pharmacy, Mianyang Central Hospital, Mianyang, Sichuan, China 621000)

机构地区：[1]四川省绵阳市中心医院药学部,四川绵阳621000

出　　处：《中国药业》2018年第8期5-8,共4页China Pharmaceuticals

摘　　要：目的建立测定人血浆中利卡西平(MHD)药物浓度的高效液相色谱(HPLC)法。方法以多索茶碱为内标,空白全血为色标,乙酸乙酯萃取血样,色谱柱为Beckman Coulter-ODS柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.3%醋酸(24∶76),流速为1.1 m L/min,柱温为30℃。紫外检测波长为240 nm。结果血浆中内源性杂质对样品及内标测定无干扰,利卡西平血药浓度在2.0~50.0μg/m L范围内与样品和内标峰高比线性关系良好,回归方程为C=31.480 2 Y-0.060 8,r=0.999 8(n=6);日内精密度的RSD<5.84%(n=5),日间精密度的RSD<6.42%(n=5);方法回收率为99.40%~103.50%,平均相对误差介于-0.60%~3.50%;检测限为0.5μg/m L。结论该方法操作简便,检测灵敏、准确,可用于利卡西平常规血药浓度的测定。Objective To establish an HPLC method for determination of drug concentration of licarbazepine（MHD） in human plasma.Methods With doxofylline as internal standard,drug-free whole blood as shade guide,the samples were extracted by ethyl acetate.Beckman Couher-ODS column （250 minx4.6 ram,5 txm） was adopted,the mobile phase was acetonitrile - 0.3% acetic acid（24：76）,the flow rate was 1.1 mL/min,the column temperature was 30 ℃ ,and the UV detection wavelength was 240 nm.Results The endogenous impurities in plasma had no interference to the determination of the sample and the internal standard. Theconcentration of MHD showed good linearity in the range of 2.0- 50. 0 pog/mL. The regression equation was C = 31. 480 2 Y-0. 060 8,r=0.999 8（n=6）.The intra-day RSD was less than 5.84% （n=5） and the inter-day RSD was less than 6.42% （n=5）.Themethodology recoveries were between 99.40% and 103.50%. The average relative error were between -0. 60% and 3.50%. The limitof detection was 0. 5 txg/mL. Conclusion This method is simple,sensitive and accurate,whlch can be used for routine determination ofblood concentration of MHD.

关 键 词：高效液相色谱法 利卡西平 血药浓度 杂质 

分 类 号：R969.1[医药卫生—药理学]