酪酸梭菌对胆汁淤积大鼠的肠黏膜通透性和紧密连接蛋白的影响  

Effects of Clostridium butyricum on the intestinal permeability and expression of tight junction proteins in rats with intrahepatic cholestasis

作　　者：马姝丽 李小芹 王菊平[1] MA Shuli;LI Xiaoqin;WANG Juping(Department of Gastroenterology, Zhengzhou Children＇s Hospital, Zhengzhou 450018, China)

机构地区：[1]郑州儿童医院郑州大学附属儿童医院药学部,郑州450018 [2]郑州儿童医院郑州大学附属儿童医院消化内科,郑州450018

出　　处：《实用医学杂志》2018年第6期937-940,共4页The Journal of Practical Medicine

基　　金：河南省医学科技攻关计划(编号:201503234)

摘　　要：目的探讨酪酸梭菌对肝内胆汁淤积大鼠的肠黏膜通透性和紧密连接蛋白的影响。方法将40只SD大鼠随机分为正常(Con)组、模型(Mod)组、酪梭菌低(CBL)、高(CBH)剂量组,每组10只。除正常组外,其余各组大鼠均灌胃α-荼基异硫氰酸盐复制肝内胆汁淤积模型,各组大鼠于造模当日开始灌胃相应药物,每天1次,连续5 d。末次给药后测定大鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、二胺氧化酶(DAO)的活性和总胆红素(T-BIL)的含量,观察肝组织病理学变化,Wersternblotting分析小肠组织中紧密连接蛋白Occludin和ZO-1的蛋白表达。结果与Con组比较,Mod组血清中ALT、AST、DAO及T-BIL水平显著升高,小肠组织中Occludin和ZO-1蛋白表达明显降低(P<0.05);与Mod组相比,CBL组、CBH组大鼠血清中ALT、AST、DAO及T-BIL含量明显降低,肝组织病理学明显改善,小肠组织中Occludin和ZO-1的蛋白表达增加(P<0.05)。结论酪酸梭菌对胆汁淤积大鼠肝损伤具有一定的保护作用,能改善其肠黏膜通透性,其机制可能与增加小肠组织中紧密连接蛋白的表达有关。Objective To investigate the effect of Clostridium butyricum on the intestinal permeabilityand expression of tight junction proteins in rats with intrahepatic cholestasis. Methods Forty SD rats were random-ly divided into normal control group（Con）,model group（Mod）,group with low（CBL）and high dose（CBH）ofClostridium butyricum. Animal models of intrahepatic cholestasis were induced by alpha-naphthylisothiocyanate.Then different drugs were administrated via oral gavage for 7 days after modeling. After the last administration,theactivities of serum alanin aminotransferase（ALT）,aspartate aminotransferase（AST）,diamineoxidase（DAO）andthe contents of total bilirubin（T-BIL）in serum were detected. Pathological changes of liver tissue were observed.The expression level of Occludin and ZO-1 in small intestine was analysed by Western blot. Results Comparedwith the normal control group,the levels of ALT,AST,DAO,and T-BIL in mordel group were significantly in-creased. The expression of Occludin and ZO-1 in small intestine was reduced（P〈0.05）. Compared with themordel group,the levels of ALT,AST,DAO,and T-BIL and the expression of Occludin and ZO-1 decreased sig-nificantly（P〈0.05）and the liver pathologic changes were improved in CBL and CBH group. Conclusions Clos-tridium butyricum can protect the liver and improve the intestinal permeability in rats with intrahepatic cholestasis,and he mechanism may be related with upregulation of Occludin and ZO-1.

关 键 词：酪酸梭菌 胆汁淤积 肠道屏障 紧密连接 

分 类 号：R722.1[医药卫生—儿科]