水中不同形态汞的高效液相色谱-原子荧光光谱测定法  

Determination of mercury species in water by high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry

作　　者：商律 梅勇 姬晓宇 梅津 SHANG Lu;MEI Yong;JI Xiao-yu;MEI Jin(Hubei Province Key Laboratory of Occupational Hazard Identification and Control, School of Public Health, Wuhan University of Science and Technology, Wuhan, Hubei 430065, Chin)

机构地区：[1]武汉科技大学医学院公共卫生学院职业危害识别与控制湖北省重点实验室,湖北武汉430065

出　　处：《环境与健康杂志》2018年第1期84-86,共3页Journal of Environment and Health

摘　　要：目的建立水中不同形态汞的高效液相色谱-原子荧光光谱测定方法。方法水样先初步过滤,进样时再经一次性水系针式滤膜过滤,水中二价汞、甲基汞和乙基汞通过C18反相色谱柱分离,经紫外线在线消解后在氢化物发生器中还原成蒸气汞,以氩气作载气,将蒸气汞从母液中分离后进行荧光光谱分析。保留时间定性,峰面积定量。结果二价汞、甲基汞和乙基汞3种形态汞标准曲线的线性范围分别为2.00~10.00μg/L、2.20~11.00μg/L、2.24~11.22μg/L;相关系数均大于0.999;检出限分别为0.21、0.64、0.72μg/L;平均加标回收率为91.21%~109.33%;RSD为2.52%~6.36%。结论该方法前处理步骤简单,方法准确,精密度可靠,适用于水样中不同形态汞的测定。Objective To establish a method for the determination of mercury species in water by high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry. Methods Water samples were preliminarily filtered by suction filtration after centrifugation and then filtered through cellulose acetate membrane filter before injection. Mercury of different species in water was separated by C18 reversed phase column for HPLC. Mercury vapor was produced in hydride generator after UV on-line digestion, argon as carrier gas and mercury vapor evaporated from the mother liquor to atomic fluorescence spectrometer. The qualitation and quantitation were confirmed according to the retention time and the peak area. Results The linearity range were 2.00-10.00μg/L, 2.20-11.00μg/L, 2.24-11.22μg/L for bivalent mercury, methylmercury and ethylmercury, respectively. And the correlation coefficients of the method were more than 0.999. The recoveries of the method were 91.21%-109.33%, the RSDs were 2.52%-6.36%. Conclusion The method is accurate and precise, the pretreatment processes are simple. The method is suitable for the monitoring of mercuric species in water.

关 键 词：不同形态的汞 水 高效液相色谱 氢化物发生 原子荧光光谱 

分 类 号：O657.3[理学—分析化学]