鼻息肉基因启动子甲基化谱的研究  

The exploration of gene promoter methylation profiling in nasal polyp

作　　者：白伟良[1] 谭海燕[1] 周倩[1] 王枭维 李进兴 BAI Weiliang;TAN Haiyan;ZHOUQian;WANG Xiaowei;LI Jinxing(Department of Otorhinolaryngology, Shengjing Hospital, China Medical University, Shenyang, 110004, China)

机构地区：[1]中国医科大学附属盛京医院耳鼻喉科,沈阳110004

出　　处：《临床耳鼻咽喉头颈外科杂志》2018年第8期599-602,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：辽宁省科学事业公益研究基金(No:2013001010);沈阳市科学技术计划(No:F11-264-1-07;F13-220-9-21)

摘　　要：目的:研究鼻息肉的基因启动子甲基化表达谱的情况,探寻其与正常鼻腔黏膜之间的启动子甲基化差异。方法:提取鼻息肉及正常对照组鼻腔黏膜中的DNA,经过免疫沉淀及扩增后,将Cy3/5标记的DNA在NimbleGen公司HG18CpG启动子甲基化基因芯片上进行杂交。应用Axon GenePix 4000Bscanner进行扫描,应用NimbleScan软件进行图像分析,然后对筛选出的差异基因进行信号通路分析,并Real-time PCR验证。结果:在鼻腔黏膜或鼻息肉组织中检测出存在CpG岛高甲基化状态的基因3 010个,其中正常鼻腔黏膜组织甲基化阴性,而鼻息肉组织有高甲基化状态的基因79个(2.62%);正常鼻腔黏膜组织基因甲基化阳性,而鼻息肉组织中甲基化阴性的基因有321个(10.66%)。正常鼻腔黏膜组织甲基化阴性,而鼻息肉组织均有高甲基化状态的基因有3个信号通路;正常鼻腔黏膜组织基因甲基化阳性,而鼻息肉组织中甲基化阴性的基因有14个信号通路。结论:基因启动子甲基化在鼻息肉发生发展中起重要作用,鼻息肉启动子甲基化谱研究为探求鼻息肉发生机制提供了有益的线索。Objective：To explore the gene promoter methylation profiles of nasal polyp,and to analysis the promoter methylation differences between the nasal polyp and the normal nasal mucosa.Method：Total DNA of the nasal polyp tissues and normal nasal mucosa were extracted.After immunoprecipitation and whole genome amplification,the DNA was labeled with Cy3/5 and hybridized in NimbleGen hybridization chamber.For array hybridization,Roche Nimblegen＇s CpG Promoter array was used.The slides were scanned using the Axon GenePix 4000 B microarray scanner.The different genes were analyzed through pathway and verified by Real-time PCR.Result：3 010 genes were found to have promoter hypermethylation in normal nasal mucosa or nasal polyp.2.62%（79/3 010）of the genes had promoter hypermethylation in all the nasal polyps,which were negative in normal nasal mucosa.10.66%（321/3 010）of the genes had promoter hypermethylation in normal nasal mucosa,which were negative in all the nasal polyps.Three pathways were found in the promoter hypermethylation of the nasal polyps.Fourteen pathways were found in the negative hypermethylation of the nasal polyps.Conclusion：Genes promoter methylation plays an important role in the development of nasal polyps,and the gene promoter methylation profiling may yield new some clues on the mechanism of nasal polyps.

关 键 词：鼻息肉 基因芯片 启动子 甲基化 

分 类 号：R765.25[医药卫生—耳鼻咽喉科]