传统蒙药苏木六味汤散的质量标准  

Study on Quality Standard for Traditional Mongolian Medicine Sumu Liuwei Tangsan

作　　者：王美丽[1] 戴丽丽[1] 春玲 田香[1] 白玉霞[1] Wang Meili;Dai Lili;Chun Ling;Tian Xiang;Bai Yuxia(Collage of Mongolian Medicine and Pharmacy, Inner Mongolia University for Nationalities, Tongliao 028300, Chin)

机构地区：[1]内蒙古民族大学蒙医药学院,通辽028300

出　　处：《世界中医药》2018年第2期472-478,共7页World Chinese Medicine

基　　金：内蒙古蒙医药工程技术研究院科学技术研究项目(GCY201508012)

摘　　要：目的:建立蒙药苏木六味汤散的质量标准。方法:采用薄层色谱(TLC)定性鉴别制剂中苏木,木香,槟榔,高良姜等;以高效液相色谱法(HPLC)测定苏木中巴西苏木素和原苏木素B的含量:色谱柱Topsil-C+(18)(4.6 mm×250 mm,5μm),流动相为乙腈-水(11∶89),检测波长为285 nm,流速1 m L/min,柱温30℃,进样量为10μL。结果:苏木,木香,槟榔,高良姜TLC图斑点清晰、专属性强;巴西苏木素与原苏木素B的进样量分别在112.875~564.375 ng、71.975~359.875 ng范围内与各自峰面积呈良好的线性关系(r分别为0.9997和0.999 9)。精密度、稳定性、重复性实验RSD%均小2.0%,加样回收率分别为99.24%、98.06%,RSD分别为0.12%、0.93%。结论:所建立的定性鉴别方法专属性强、重复性好、定量方法简便、准确、可靠,可用于传统蒙药苏木六味汤散的质量控制。Objective： To establish the quality standard of traditional Mongolian Sumu Liuwei Tangsan. Methods： Lignum Sappan,Radix Aucklandiae,Semen Arecae and Rhizoma Alpiniae Officinarum were identified by TLC qualitatively. The content of brazilin and protosappanin B in Lignum Sappan was determined by HPLC. The separation was performed on Topsil C_（18）（ 4. 6 mm × 250 mm,5 μm） with mobile phase consisted of acetonitrile-water（ 11∶ 89） at the flow rate of 1. 0 m L/min. The detection wavelength was set at 285 nm. The column temperature was 30 ℃. The sample size was 10 μL. Results： The TLC spots of Lignum Sappan,Radix Aucklandiae,Semen Arecae and Rhizoma Alpiniae Officinarum were clear and specific. The linear range was 112. 875-564. 375 ng for brazilin（ r = 0. 9997） and 71. 975-359. 875 ng for protosappanin B（ r = 0. 9999） respectively. The RSDs of precision,stability and repeatability tests were no more than 2%. The average recoveries were respectively 99. 24 %（ RSD = 0. 12 %,n = 6） and 98. 06 %（ RSD = 0. 93 %,n = 6）. Conclusion： The qualitative identification is specific and reproducible,and quantitative method is simple,accurate and reliable,which can be used for the quality control of traditional medicine Sumu Liuwei Tangsan.

关 键 词：蒙药苏木六味汤散 薄层色谱法 高效液相色谱法 巴西苏木素 原苏木素B 

分 类 号：R282.5[医药卫生—中药学]