离子交换树脂混用技术分离纯化妥布霉素  

Purification of tobramycin by mixed ion exchange resins

作　　者：牛猛 徐环昕[1,2] 宁方红 江邦和[1,2] 刘坐镇 Niu Meng;Xu Huan-xin;Ning Fang-hong;Jiang Bang-he;Liu Zuo-zhen(State Key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237;Huazhen Sei ＆ Tech Co. Ltd., Shanghai 200237)

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]上海华震科技有限公司,上海200237

出　　处：《中国抗生素杂志》2018年第4期457-461,共5页Chinese Journal of Antibiotics

摘　　要：目的开发利用离子交换树脂混用技术分离纯化妥布霉素的工艺。方法建立2,4-二硝基氟苯(DNFB)HPLC柱前衍生化检测妥布霉素及相关物质,以树脂吸附量和解吸量以及妥布霉素纯度为指标,通过静态吸附和动态吸附实验,对妥布霉素在树脂上吸附和解吸条件进行优化。结果从10种不同类型的树脂筛选出HZ-3C和JK110分离纯化妥布霉素,确定最优工艺条件如下:确定两种树脂最佳比例为3:1,上样p H值为9,上样流速为3BV/h,使用浓度0.20%和0.35%氨水溶液进行梯度洗脱。采用优化后的条件,妥布霉素纯度从32.85%提高到94.82%,回收率为88.26%。结论建立了一种新的妥布霉素分离纯化方法,优化后的工艺妥布霉素得率高、工艺简单易行,适合工业化生产。Objective To develop the separation and purification processes of tobramycin with mixed resins. Methods An HPLC method is established for the determination of tobramycin and its related substances by precolumn derivatization with 2,4-dinitrofiuorobenzene. Based on adsorption capacity and desorption capacity of resin and the purity of tobramycin, optimal conditions for adsorption and elution of tobramycin were performed with static and dynamic experiments. Results HZ-3C and JK110 were selected from ten types of resin as the effective adsorbent for separation and purification of tobramycin. The purification conditions were specified as the following： The ratio of HZ-3C and JKll0 was 3：1, the pH value was 9, the flow rate was 3BV/h. The optimum desorption conditions was obtained by gradient elution with 0.3% and 0.7% ammonia water, under these conditions, tobramycin content were increased from 32.85% to 94.82%, with a tobramycin recovery rate of 88.26%. Conclusion The method for separation and purification of tobramycin was established, it was simple and easy, and suitable for industrial production with high purity and desorption rate.

关 键 词：妥布霉素 混合树脂 纯化 

分 类 号：R978.1[医药卫生—药品]