地黄提取物对小鼠免疫功能的影响  

Effects of Rehmannia glutinosa extract on immune function of mice

作　　者：李国辉[1] 刘佳[2] 刘建伟[2] 郭莉[2] 刘聚祥[1] 何欣[1] LI Guo-hui;LIU Jia;LIU Jian-wei;GUO Li;LIU Ju-xiang;HE Xin(College of Veterinary Medicine, Hebei Agricultural University, Baoding , Hebei 071000, China;Baoding Jinong Animal Pharmaceutical Co. ,Ltd, Baoding, Hebei 071000, China)

机构地区：[1]河北农业大学动物医学学院,河北保定071000 [2]保定市冀农动物药业有限公司,河北保定071000

出　　处：《中国兽医学报》2018年第4期765-769,775,共6页Chinese Journal of Veterinary Science

基　　金：河北省现代农业产业技术体系蛋鸡产业创新团队项目;河北省优秀青年基金资助项目(YQ2013019)

摘　　要：为研究地黄提取物对小鼠免疫功能的影响，灌胃给予小鼠地黄提取物，通过免疫器官指数、小鼠碳粒廓清指数、白细胞总数，流式细胞仪检测NK细胞活性，ELISA试剂盒检测血清中IL-2、TNF—a生成水平评价地黄提取物对小鼠非特异性免疫的影响；通过流式细胞仪检测外周血T淋巴细胞亚群（CD4^＋T／CD8^＋T），MTT法检测脾淋巴细胞增殖水平、血清溶血素抗体生成水平以及血清中IgG、IgA含量评价地黄提取物对小鼠特异性免疫的影响。结果显示，地黄提取物可显著提高免疫器官指数，提高碳粒廓清指数，IL-2和TNF—α水平也明显提高。T淋巴细胞比值、血清溶血素水平及脾淋巴细胞增殖率显著提高；地黄提取物不同剂量组小鼠血清IgG、IgA水平也有不同程度地提高。结果表明，地黄提取物能促进小鼠免疫功能。The study was conducted to investigate the effect of Rehmannia glutinosa extract on im- mune function of mice. The spleen index,the carbon clearance index,the total leucocytes,the activ- ity of NK cell,IL-2 and TNF-a content in mouse serum by the methods of ELISA were used to es- timate the non-specific immunity function. T lymphocyte subsets（CD4＋ T/CD8＋ T）, the prolifera- tion of splenic lymphocytes, the content of serum hemolysin and the immunoglobulin contents （IgG,IgA） in mouse serum by the methods of ELISA were used to estimate the non-specific im- munity function. The results showed that the spleen index and the IgG, IgA contents were in- creased. The IL-2 and TNF-acontent in mouse serum and the total leucocytes were increased in middle and high dose. T lymphocyte subsets（CD4＋ T /CD8＋ T） and the the content of serum he- molysin were increased in high dose. It is concluded that Rehmannia glutinosa extract could in- creased the immune function of mice.

关 键 词：地黄提取物 免疫调节 小鼠 

分 类 号：S853.74[农业科学—临床兽医学] S852.4[农业科学—兽医学]