奶牛乳腺上皮细胞中Bta-miR-877靶基因的初步验证  

Validation of Bta-miR-877 target genes in bovine mammary epithelial cells

作　　者：杨雨薇[2] 房希碧[2] 于海滨[2] 姜平[2] 杨润军[2] 赵志辉 YANG Yu-wei;FANG Xi-bi;YU Hal-bin;JIANG Ping;YANG Rui-jun;ZHAO Zhi-hui(Agricultural College, Guangdong Ocean University, Zhanjiang , Guangdong 524088, China;College of Animal Science ,Jilin University ,Changchun 130062 ,China)

机构地区：[1]广东海洋大学农学院,广东湛江524088 [2]吉林大学动物科学学院,吉林长春130062

出　　处：《中国兽医学报》2018年第4期809-813,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31372278,31672389);国家“863”计划资助项目(2013AA102505);吉林省省级产业技术研究和发展资助项目(2016C032);吉林省科技发展计划资助项目(20170519014JH)

摘　　要：利用高通量测序的方法筛选出在高脂奶牛和低脂奶牛中差异表达的miR-877和其预测的靶基因。通过生物信息学方法预测出edn1、ptgis可能是miR877的靶基因，之后通过荧光定量检测miR877以及其靶基因的表达量。结果显示，转染miR877mimics组miR-877的相对表达量显著高于转染miR-877inhibitor组；靶基因的相对表达量，转染miR877mimics组的ednl、ptgis基因表达水平均显著低于转染miR-877inhibitor组（P〈0．01）。经Graph—PadPrism6分析显示，edn1、ptgis基因的相对表达量与miR-877的表达量呈负相关，初步验证edn、ptgis是miR-877的靶基因。本试验为深入了解miR-877在乳脂质代谢调节中的作用提供依据，从而为生产实践提供一定的理论基础。High-throughput sequencing was used to screen the ditterentially expression if miR-877 and predict its target genes in high-fat and low-fat dairy cows. ednl and ptgis might be the target genes of miR-877 using bioinformatics method analysis,then the expression of miR-877 and its tar- get genes were detected by qPCR. The results showed that the expression of miR-877 was signifi- cantly higher in miR-877 mimics group than miR-877 inhibitor group. The expression levels of ednl and ptgis genes were significantly lower in cells those of transfected miR-877 mimics than miR-877 inhibitor group（P〈0.01）. The relative expression of ednl and ptgis genes were nega- tively correlated with the expression level of miR-877 by Graph Pad Prism6 analysis. It was pre- liminarily verified that edn and ptgis were the target genes of miR-877. It provided a theoretical basis and guidance for production practice in the regulation of lipid metabolism.

关 键 词：奶牛乳腺上皮细胞 miR-877 edn1 ptgis 

分 类 号：S823.91[农业科学—畜牧学] Q78[农业科学—畜牧兽医]