硫辛酸对奥硝唑所致少弱精子症大鼠精子发生的保护作用研究  

Lipoic acid protects spermatogenesis in male rats with ornidazole-induced oligoasthenozoospermia

作　　者：张国巍 万秀霞[2] 万长春[3] 李凯强 李奕泽 翁志强 商学军 ZHANG Guo-wei;WAN Chang-chun;LI Kai-qiang;LI Yi-zel;WENG Zhi-qiang;SHANG Xue-jun(Department of Andrology, 4. Department of Outpatients, Nanjing School of Clinical Medicine, Southern Medical University / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China;Virtual La- boratory Section, Laboratory of Human Integrated Biological Functions, School of Basic Medicine, Qingdao University, Qingdao, Shandong 266071, China;Department of Clinical Laboratory, Jinhu People＇s Hospital, Jinhu, Jiangsu 211600, China)

机构地区：[1]南方医科大学南京临床医学院/南京军区南京总医院男科,江苏南京210002 [2]青岛大学基础医学院人体机能学实验室虚拟实验室,山东青岛266071 [3]金湖县人民医院检验科,江苏金湖211600 [4]南方医科大学南京临床医学院/南京军区南京总医院门诊部,江苏南京210002

出　　处：《中华男科学杂志》2018年第4期297-303,共7页National Journal of Andrology

基　　金：国家自然科学基金(81370750;81771646);2017年度军队计生专项研究任务计划(17JS013);江苏省"六大人才高峰"资助(WSN-069)~~

摘　　要：目的:研究硫辛酸(LA)对奥硝唑(ORN)所致少弱精子症模型雄性大鼠生精功能的保护作用。方法:取70只雄性SD大鼠,随机分成7组,每组10只,分别为:A组(溶剂对照组):1 ml 0.5%羧甲基纤维素钠(CMC-Na)+1 ml橄榄油;B组(低剂量ORN造模组):400 mg/kg ORN+1 ml橄榄油;C组(低剂量ORN+低剂量LA治疗组):400 mg/kg ORN+50 mg/kg LA;D组(低剂量ORN+高剂量LA治疗组):400 mg/kg ORN+100 mg/kg LA;E组(高剂量ORN造模组):800 mg/kg ORN+1 ml橄榄油;F组(高剂量ORN+低剂量LA治疗组):800 mg/kg ORN+50 mg/kg LA;G组(高剂量ORN+高剂量LA治疗组):800 mg/kg ORN+100 mg/kg LA。连续给药20 d,处死大鼠,称量大鼠体重、睾丸、附睾、精囊重量,计算脏器指数,检测附睾精子计数及活力,睾丸、附睾做HE染色观察组织形态学改变。结果:与A组相比,E组大鼠体重增量、睾丸、附睾脏器指数明显减少[(117.67±11.53)g vs(88.11±12.65)g;(1.06±0.12)%vs(0.65±0.13)%;(0.21±0.03)%vs(0.17±0.01)%,P均<0.01];与E组相比,F组大鼠附睾脏器指数明显增加[(0.17±0.01)%vs(0.20±0.02)%,P<0.01],G组大鼠体重增量、睾丸、附睾脏器指数明显增加[(88.11±12.65)g vs(102.70±16.10)g,P<0.05;(0.65±0.13)%vs(0.95±0.06)%,P<0.01;(0.17±0.01)%vs(0.19±0.02)%,P<0.05],精囊脏器指数各组之间并无明显差异。与A组相比,B组大鼠精子活力明显降低[(74.12±8.73)%vs(40.25±6.08)%,P<0.01],E组大鼠精子计数与活力明显降低[(38.59±6.40)×105/100 mg vs(18.67±4.59)×105/100 mg;(74.12±8.73)%vs(27.58±8.43)%,P均<0.01];与B组相比,C、D组大鼠精子活力明显增加[(40.25±6.08)%vs(58.13±7.62)%;(40.25±6.08)%vs(76.04±8.44)%,P均<0.01];与E组相比,F、G组大鼠精子计数及活力明显增加[(18.67±4.59)×10~5/100 mg vs(25.63±9.66)×10~5/100 mg,P<0.05;(18.67±4.59)×10~5/100 mg vs(29.92±4.15)×10~5/100 mg,P<0.01;(27.58±8.43)%vs(36.56±11.08)%,P<0.05;(27.58±8.43)%vs(45.05±9.59)%,P<0.01]。A、B、C、D组大鼠睾丸、附睾组织形态学无明显改变。E组大鼠睾丸Objective： To study the protective effect of lipoic acid （LA） on the spermatogenic function of the male rats with oligoasthenozoospermia induced by ornidazole （ORN）. Methods： Seventy male SD rats were equally randomized into groups A （sol- vent control： 1 ml 0.5% CMC-Na ± 1 ml olive oil）, B （low-dose ORN model： 400 mg/kg ORN suspension ± 1 ml olive oil）, C （low-dose ORN ± low-dose LA treatment： 400 mg/kg ORN ± 50 mg/kg LA）, D （low-dose ORN ± high-dose LA treatment： 400 mg/kg ORN ± 100 mg/kg LA）, E （high-dose ORN model： 800 mg/kg ORN suspension ± 1 ml olive oil）, F （high-dose ORN ± low-dose LA treatment： 800 mg/kg ORN ± 50 mg/kg LA）, and G （high-dose ORN ± high-dose LA treatment： 800 mg/kg ORN ± 100 mg/kg LA）, and treated respectively for 20 successive days. Then all the rats were sacrificed and the weights of the body, tes- tis, epididymis and seminal vesicle obtained, followed by calculation of the organ index, determination of epididymal sperm concentra- tion and motility, and observation of the histomorphological changes in the testis and epididymis by HE staining. Results ： Compared with group A, group E showed significantly decreased body weight （ [ 117.67 ± 11.53 ] vs [ 88.11 ± 12.65 ] g, P 〈 0.01 ） and in- dexes of the testis （[1.06 ± 0.12] vs [0.65 ± 0.13] %, P 〈 0.01） and epididymis （[0.21 ± 0.03] vs [0.17 ± 0.01] %, P 〈 0.01 ）. In comparison with group E, group F exhibited remarkable increases in the epididymal index （ [0.17 ± 0.01 ] vs [0.20 ± 0.02] %, P 〈 0.01）, and so did group G in the body weight （[88.11 ± 12.65] vs [102.70 ± 16.10] g, P 〈 0.05） and the indexes of the testis （[0.65 ± 0.13] vs [0.95±0.06] %, P 〈 0.01） and epididymis （[0.17 ± 0.01] vs [0.19 ± 0.02] % , P 〈 0.05）, but no obvious difference was observed in the index of seminal vesicle among different groups. Compared with group A, group B manifested significant decreases in sperm motility （ [74.12 ± 8.73] vs [

关 键 词：硫辛酸 奥硝唑 少弱精子症 生精功能 

分 类 号：R698.2[医药卫生—泌尿科学]