低MCV引起PLT计数假性增高的实验研究  

Analysis of the Influence of Different MCV Level on the Platelet False Increase Counts

作　　者：荆晶 胡恩亮 樊爱琳 郑善銮 郝晓柯 JING Jing;HU En-liang;FAN Ai-lin;ZHENG Shan-luan;HAO Xiao-ke(Department of Clinical Laboratory, Xijing Hospital ,the Air Force Military Medical University ,Xi＇ an 710032 ,China)

机构地区：[1]空军军医大学西京医院检验科,西安710032

出　　处：《现代检验医学杂志》2018年第2期108-110,共3页Journal of Modern Laboratory Medicine

摘　　要：目的评价低红细胞平均体积(MCV)对PLT计数的影响,为实验室制定PLT复检规则提供依据。方法随机收集2017年3~8月期间的466例门诊检测血细胞计数及五分类患者EDTA抗凝血标本,检验结果分为三组:A组MCV≤65fl,B组65fl<MCV≤70fl,C组70fl<MCV≤75fl。采用SYSMEX-XN9000血细胞分析仪分别进行电阻抗法(PLT-I)和荧光核酸染色法(PLT-F)血小板计数,将两种方法检测的血小板计数结果进行统计分析。结果A组PLT-I值=(322.8±109.1)×109/L,PLT-F值=(282.60±100.5)×109/L,二者之间差异有统计学意义(t=6.799,P<0.05);B组PLT-I值=(305.7±111.7)×109/L,PLT-F值=(304.8±112.3)×109/L;C组PLT-I值=(292.2±84.4)×109/L,PLTF值=(291.6±84.4)×109/L。结论当存在小红细胞或红细胞碎片的情况下,即低MCV≤65fl时,电阻抗法(PLT-I)检测PLT结果,会引起PLT计数的假性增高。Objective To evaluate the influence of the mean corpuscular volume（MCV）in different ranges on PLT counts,provide the theoretical basis for making PLT counts review rules in laboratory.Methods From March 2016 to August 2016 in Xijing Hospital department of outpatient who perform complete blood cell count were randomly divided into 3 groups,MCV≤65 fl in A group,65 flMCV≤70 fl in B group and 70 flMCV≤75 fl in C group.The reference method（Coulter principle）compared with the fluorescence method,accuracy analysis of different monitoring methods of counting PLT.Results PLT-I and PLT-F count values in A group was（322.8±109.1）×109/L and（282.60±100.5）×109/L respectively,and there was significant differences between the two groups（t=6.799,P〈0.05）.In B group,the count values was（305.7±111.7）×109/L and（304.8±112.3）×109/L respectively,and there was no differences between two groups.In C group,the count values was（292.2±84.4）×109/L and（291.6±84.4）×109/L respectively,and there was no differences between two groups neither.Conclusion When small red blood cells or blood cell debris present in blood circulation,MCV≤65 fl,the reference testing（Coulter principle）of platelets causes a false increase in platelet count.

关 键 词：血小板计数 小红细胞 红细胞碎片 红细胞平均体积 

分 类 号：R446.111[医药卫生—诊断学]