两株H6亚型禽流感病毒全基因组序列测定及遗传进化分析  

Genome sequencing and genetic analysis of H6N2 and H6N6 subtype avian influenza viruses isolated from ducks

作　　者：李旭勇[1] 李玉保[1] 刘文强[1] 马树杰[2] 崔鹏飞[2] 孙香雪 潘玉迪 陈化兰[2] 郭晶[1] LI Xu-yong;LI Yu-bao;LILT Wen-qiang;MA Shu-jie;CUI Peng-fei;SUN Xiang-xue;PAN Yu-di;CHEN Hua-lan;GUO Jing(College of Agricultural, Liaocheng University, Liaocheng, Liaocheng 252000, China;State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China)

机构地区：[1]聊城大学农学院,山东聊城252000 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150069

出　　处：《中国预防兽医学报》2018年第4期275-278,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：兽医生物技术国家重点实验室开放基金(SKLVBF201707);山东省自然科学基金(ZR2017BC057)

摘　　要：为研究2016年在活禽交易市场鸭体内分离的2株H6亚型禽流感病毒(AIV)[分别是A/duck/Jiangxi/88/2016(H6N6)(简称DK/88/16)和A/duck/Jiangxi/219/2016(H6N2)(简称DK/219/16)]分子特征及遗传进化规律,本实验对其进行了全基因组扩增和测序,并进行了遗传进化分析。结果显示,2株病毒间HA基因同源性较低,仅为94.5%,但与我国近年分离的H6亚型病毒株保持较高同源性。DK/88/16和DK/219/16的NA基因分别与我国近年流行的流感病毒N6和N2基因保持较高同源性。对2株病毒的内部基因分析显示,其内部基因的来源较为复杂,其中DK/88/16的内部基因PA来源于我国最近流行的H5N2或H5N6病毒,显示该株病毒为新的重组病毒。对2株病毒的分子特征分析显示,除M1蛋白的30和215位点及NS1蛋白的42位点具有增强病毒对小鼠致病力的分子特征外,其余均保持低致病力分子特征。以上结果表明,H6N2和H6N6亚型AIV在我国鸭群中持续存在,并不断的遗传重组,需要进行持续的流行病学监测。Two H6 subtype influenza viruses were isolated from ducks during the viral surveillance, 2016, including duck/Jiangxi/88/2016 （H6N6） （DK/88/16） and duck/Jiangxi/219/2016 （H6N2） （DK/219/16）. To investigate the molecular characteristics and the evolution of the H6 subtype influenza virus, the eight viral gene segments were amplified and the whole genome of the H6N2 and H6N6 viruses used in this study were sequenced. According to the genetic analysis, the two viruses showed only 94.5% identity in their HA gene, but they showed high similarity with the HA genes of H6 viruses isolated in recent years. The N6 and N2 genes of DK/88/16 and DK/219/16 shared high similarity with that of the viruses isolated in recent years. The source of the internal gene segments in the two AIVs were complicated, e.g. the PA gene of DK/88/16 was originated from H5N2 or H5N6 viruses, which indicated the virus was a new reassortant. The two viruses showed low pathogenicity to mammals according to the molecular analysis of the key amino acids. These results indicat that the H6 subtype viruses are continuously evolving in ducks, resulting in the emergence of the new reassortants, which will urge us to perform the persistent viral surveillance.

关 键 词：禽流感病毒 H6亚型 基因遗传分析 

分 类 号：S852.65[农业科学—基础兽医学]