童心康合剂对实验性自身免疫性心肌炎大鼠心肌纤维化的影响  

Effect of Tongxinkang mixture on myocardial fibrosis in rats with experimental autoimmune myocarditis

作　　者：宋惠霄[1] 刘艳红 陈鲁[1] 李燕宁[1] 魏洪伟[2] SONG Hui-xiao;LIU Yan-hong;CHEN Lu;LI Yan-ning;WEI Hong-wei(Affiliated Hospital of Shan dong University of Traditional Chinese Medicine, Shandong Jinan 250011 ,China;Jinan Third People＇s Hospital,Shandong Ji nan 250101, China)

机构地区：[1]山东中医药大学附属医院,山东济南250011 [2]济南市第三人民医院,山东济南250101

出　　处：《中国医院药学杂志》2018年第6期610-614,共5页Chinese Journal of Hospital Pharmacy

摘　　要：目的:探讨童心康合剂对实验性自身免疫性心肌炎(EAM)模型大鼠心肌纤维化的影响。方法:将60只Lewis大鼠随机分为正常对照组、EAM模型组、玉丹荣心丸组、童心康合剂高、中、低剂量组,每组10只。于免疫56d处死存活大鼠,观察心肌病理变化、Masson染色观察心肌纤维化,免疫组化测心肌组织金属蛋白酶-9(MMP-9)及其组织抑制物-1(TIMP-1)的表达。结果:童心康合剂大中剂量组与玉丹荣心丸组心肌病理积分均低于EAM模型组(P<0.05),3组之间差异无显著性(P>0.05),童心康合剂小剂量组与EAM模型组病理积分差异无显著性(P>0.05)。童心康合剂大中剂量、玉丹荣心丸均能抑制心肌纤维化,童心康合剂大中剂量均优于玉丹荣心丸(P<0.05),且2组之间差异无显著性(P>0.05)。童心康合剂中剂量能上调心肌组织MMP-9的表达,下调其TIMP-1的表达。结论:童心康合剂大中剂量、玉丹荣心丸可减轻EAM模型大鼠心肌炎症,抑制心肌纤维化,此作用可能与调节MMP-9/TIMP-1平衡有关。OBJECTIVE To study the effect of Tongxinkang mixture on myocardial fibrosis in rats with experimental autoimmune myocarditis（EAM）.METHODS Sixty Lewis rats were randomly divided into 6 groups,including normal group,EAM group,Yudanrongxin pill group,high dose,medium dose and low dose Tongxinkang mixture groups.Each group had 10 rats.The survived rats were killed after 56 days,and then myocardial pathological changes,myocardial fibrosis and MMP-9/TIMP-1 expression were observed.RESULTS The myocardial pathological scores of high dose and medium dose Tongxinkang mixture group,and Yudanrongxin pill group were significantly lower than those of low dose Tongxinkang mixture group and EAM group（P0.05）.High dose and medium dose Tongxinkang mixture could more significantly inhibit myocardial fibrosis than Yudanrongxin pill（P0.05）.Medium dose Tongxinkang mixture could upregulate the expression of MMP-9 and downregulate the expression of TIMP-1.CONCLUSION This research shows that Tongxinkang mixture can reduce myocarditis in EAM rats.Tongxinkang mixture can protect myocardium and suppress myocardial fibrosis by regulating the expression balance of MMP-9 and TIMP-1.

关 键 词：自身免疫性心肌炎 童心康合剂 MMP-9/TIMP-1 

分 类 号：R96[医药卫生—药理学]