连续流动法测定烟草中还原糖含量的方法优化  

Optimization of a continuous flow method to determine reducing sugar content in tobacco

作　　者：何声宝[1] 罗安娜[1] 王英元[1] 冯晓民[1] 张威[1] 范月月 刘楠[1] HE Shengbao;LUO Anna;WANG Yingyuan;FENG Xiaomin;ZHANG Wei;FAN Yueyue;LIU Nan(China National Tobacco Quality Supervision and Test Center, Zhengzhou 450001, China)

机构地区：[1]国家烟草质量监督检验中心

出　　处：《烟草科技》2018年第4期41-45,共5页Tobacco Science & Technology

基　　金：中国烟草总公司科技重大专项项目"提高细支卷烟质量稳定性的关键工艺技术研究"(522016AA0190.2)

摘　　要：为提高连续流动法测定烟草样品中还原糖含量的结果准确性,采用AA3连续流动分析仪对烟草行业标准YC/T 159—2002中的测定条件进行了优化。优化后的条件为:红/红管路中的活化水调整为0.20mol/L盐酸溶液、氢氧化钠溶液浓度降至0.35 mol/L、加热槽温度降至79℃。结果表明:(1)方法改进后,标准曲线的相关系数为0.999 9,检出限和定量限分别为0.006 3%和0.021 0%,日内和日间相对标准偏差均小于5%,加标回收率在96.88%~102.86%之间。(2)经F检验,改进方法与标准方法的测定结果间无显著差异,且使用改进后的方法测定烟草中还原糖含量时,能连续准确测定60杯,比原方法增加40杯。In order to improve the accuracy to determine reducing sugar contents in tobacco by continuous flow method, the test parameters described in the domestic tobacco industrial standard YC/T 159--2002 were optimized via an AA3 continuous flow analyzer. The optimized conditions were shown as follows： 0.20 mol/L hydrochloric acid solution was used as the activated water in red/red pipeline, the concentration of sodium hydroxide solution was reduced to 0.35 mol/L, and the temperature of the heating tank was lowered to 79 ~2. The results showed that： 1） Adopting the optimized method, the correlation coefficient of the standard curve was 0.999 9, the limit of detection and the limit of quantitation were 0,006 3% and 0.021 0%, respectively. The relative standard deviations （RSDs） of intra- and inter-day were less than 5% and the spiked recoveries ranged from 96.88% to 102.86%. 2） F test indicated that there was no significant difference between the results determined by the optimized method and the standard method. The optimized method could continuously and accurately determine the content of reducing sugar in tobacco by 60 cups （40 cups more than the standard method）.

关 键 词：连续流动法 烟草 还原糖 方法优化 

分 类 号：TS411.1[农业科学—烟草工业]