一种Hg^(2+)荧光探针的合成及其在活细胞成像中的应用（英文）  

A Hg^(2+)-Selective Fluorescence Probe: Synthesis and Application in Aqueous Solution and Living Cell Imaging

作　　者：张敏[1] 肖慧丰 韩志湘 仰榴青[1] 吴向阳 Zhang Min;Xiao Huifeng;Han Zhixiangb;Yang Liuqinga;Wu Xiangyang(School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013;School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013)

机构地区：[1]江苏大学化学化工学院,镇江212013 [2]江苏大学环境与安全工程学院,镇江212013

出　　处：《有机化学》2018年第4期926-930,共5页Chinese Journal of Organic Chemistry

基　　金：Project supported by the Natural Science Foundation of Jiangsu Province(No.BK20140536)~~

摘　　要：设计合成了一个新的罗丹明席夫碱类荧光探针1,其结构经~1H NMR、^(13)C NMR、MS和X射线单晶衍射的验证.通过紫外-可见光谱法和荧光光谱法研究了探针1在乙醇-水(V/V=7/3,HEPES 10 mmol/L,p H=7.0)中对Hg^(2+)的识别性能.探针1通过显著的荧光增强来识别Hg^(2+),并具有良好的选择性和抗干扰能力.通过Job’s plot和MS证明了探针1和Hg^(2+)形成1∶1的配合物.探针1的荧光强度与Hg^(2+)浓度(0~50μmol/L)呈良好的线性关系,可以定量地检测该范围内的Hg^(2+).在MGC-803活细胞中的荧光显微成像表明,探针1可检测生物体内的Hg^(2+).A novel rhodamine B Schiff-base fluorescence probe 1 was synthesized and characterized by 1H NMR, 13C NMR, MS and single-crystal X-ray analysis. The recognition properties of the probe 1 with Hg2＋ ions were investigated in ethanol-water （V/V： 7/3, HEPES 10 retool/L, pH ： 7.0） by the UV-Vis spectrophotometry and the fluorescence spectrophotometry. The probe 1 showed remarkable ＂turn-on＂ fluorescent responses to Hg2＋ with good selectivity over the other metal ions examined. The 1 ： 1 binding model of probe 1 with Hg2＋ was established by Job＇s plot and MS spectra. The probe can be applied to the quantitative analysis of Hg2＋ with a linear range covering from 0 to 50 μmot/L. The fluorescence imaeine exoeriments in MGC-803 living, cells demonstrated that 1 could be used in biological systems to monitor Hg2＋.

关 键 词：罗丹明B 合成 荧光探针 HG^2+ 生物成像 

分 类 号：O657.3[理学—分析化学] Q2-33[理学—化学]