The formation of fibers via complementary base pairing of DNA-conjugated bovine serum albumin  

作　　者：Jing Wei Si Sun Qian Lu Pengcheng Gao Yang Wang Xianhe Li Yong Jiang 

机构地区：[1]School of Chemistry and Chemical Engineering, Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research, Southeast University

出　　处：《Chinese Chemical Letters》2018年第3期461-463,共3页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(NSFC, No. 21174029);the Industry Academia Cooperation Innovation Fund of Jiangsu Province(No.BY201412707);the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD);the Fundamental Research Funds for the Central Universities (No. 2242016K41020)

摘　　要：Assembled protein-based substances are emerging and promising classes of materials that provide unique properties for various applications in biotechnology and nanotechnolegy. Self-assembly is an effective way to immobilize protein. In this study, DNAs-conjugated bovine serum albumin （BSA） assembled into fibers via DNA hybridization is demonstrated. The morphology of fibers was observed by optical microscopy, scanning electron microscopy （SEM）, and atomic force microscopy （AFM）, and the assembly mechanism was then analysed and discussed. BSA molecules were first linked by DNA molecule and formed linear chains. These chains then were parallelly linked through additional DNA hybridization. Finally, several BSA chains further assembled into fibers by layering lamellae in a parallel manner. This work perhaps will provide a guide to the immobilization of enzyme, which could be applied to increase its catalytic efficiency in biomedicine and nanotechnology.Assembled protein-based substances are emerging and promising classes of materials that provide unique properties for various applications in biotechnology and nanotechnolegy. Self-assembly is an effective way to immobilize protein. In this study, DNAs-conjugated bovine serum albumin （BSA） assembled into fibers via DNA hybridization is demonstrated. The morphology of fibers was observed by optical microscopy, scanning electron microscopy （SEM）, and atomic force microscopy （AFM）, and the assembly mechanism was then analysed and discussed. BSA molecules were first linked by DNA molecule and formed linear chains. These chains then were parallelly linked through additional DNA hybridization. Finally, several BSA chains further assembled into fibers by layering lamellae in a parallel manner. This work perhaps will provide a guide to the immobilization of enzyme, which could be applied to increase its catalytic efficiency in biomedicine and nanotechnology.

关 键 词：Assembling DNA Protein Microscope Atomic force microscope 

分 类 号：O641.3[理学—物理化学]