The Hardy Rubber Tree Genome Provides Insights into the Evolution of Polyisoprene Biosynthesis  

作　　者：Ta-na Wuyun Lin Wang Huimin Liu Xuewen Wang Liangsheng Zhang Jeffrey L. Bennetzen Tiezhu Li Lirong Yang Panfeng Liu Lanying Du Lu Wang Mengzhen Huang Jun Qing Iili Zhu Wenquan Bao Hongguo Li Qingxin Du Jingle Zhu Hong Yang Shuguang Yang Hui Liu Hui Yue Jiang Hu Suoliang Yu Yu Tian Fan Liang Jingjing Hu Depeng Wang Ruiwen Gao Dejun Li Hongyan Du 

机构地区：[1]Non-timber Forest Research and Development Center, Chinese Academy of Forestry, Zhengzhou 450003, China [2]Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of TropicalAgricultural Sciences, Danzhou 571737, China [3]The Eucommia Engineering Research Center of State Forestry Administration, Zhengzhou 450003, China [4]Center for Genomics and Biotechnology State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Fujian Agriculture and Forestry University,Fuzhou 350002, China [5]Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Zhengzhou 450003, China [6]Shandong BELO EUCOMMIA Biological Engineering Co., Ltd., Qingzhou 262500, China [7]Nextomics Biosciences Co., Ltd., Wuhan 430073, China [8]Wuhan Unique Gene Bioinformatics Science and Technology Co., Ltd., Wuhan 430073, China [9]Department of Genetics, University of Georgia, Athens, GA 30602, USA [10]These authors contributed equally to this article.

出　　处：《Molecular Plant》2018年第3期429-442,共14页分子植物（英文版）

摘　　要：Eucommia ulmoides, also called hardy rubber tree, is an economically important tree; however, the lack of its genome sequence restricts the fundamental biological research and applied studies of this plant species. Here, we present a high-quality assembly of its ~l.2-Gb genome （scaffold N50 = 1.88 Mb） with at least 26 723 predicted genes for E. ulmoides, the first sequenced genome of the order Garryales, which was obtained using an integrated strategy combining Illumina sequencing, PacBio sequencing, and BioNano mapping. As a sister taxon to lamiids and campanulids, E. ulmoides underwent an ancient genome triplication shared by core eudicots but no further whole-genome duplication in the last ~125 million years. E. ulmoides exhibits high expression levels and/or gene number expansion for multiple genes involved in stress responses and the biosynthesis of secondary metabolites, which may account for its considerable environmental adaptability. In contrast to the rubber tree （Hevea brasiliensis）, which produces cis-polyisoprene, E. ulmoides has evolved to synthe- size long-chain trans-polyisoprene via farnesyl diphosphate synthases （FPSs）. Moreover, FPS and rub- ber elongation factor/small rubber particle protein gene families were expanded independently from the H. brasiliensis lineage. These results provide new insights into the biology of E. ulmoides and the origin of polyisoprene biosynthesis.Eucommia ulmoides, also called hardy rubber tree, is an economically important tree; however, the lack of its genome sequence restricts the fundamental biological research and applied studies of this plant species. Here, we present a high-quality assembly of its ~l.2-Gb genome （scaffold N50 = 1.88 Mb） with at least 26 723 predicted genes for E. ulmoides, the first sequenced genome of the order Garryales, which was obtained using an integrated strategy combining Illumina sequencing, PacBio sequencing, and BioNano mapping. As a sister taxon to lamiids and campanulids, E. ulmoides underwent an ancient genome triplication shared by core eudicots but no further whole-genome duplication in the last ~125 million years. E. ulmoides exhibits high expression levels and/or gene number expansion for multiple genes involved in stress responses and the biosynthesis of secondary metabolites, which may account for its considerable environmental adaptability. In contrast to the rubber tree （Hevea brasiliensis）, which produces cis-polyisoprene, E. ulmoides has evolved to synthe- size long-chain trans-polyisoprene via farnesyl diphosphate synthases （FPSs）. Moreover, FPS and rub- ber elongation factor/small rubber particle protein gene families were expanded independently from the H. brasiliensis lineage. These results provide new insights into the biology of E. ulmoides and the origin of polyisoprene biosynthesis.

关 键 词：Eucommia ulmoides genome environmental adaptability eucommia rubber 

分 类 号：S794.1[农业科学—林木遗传育种] Q343.2[农业科学—林学]