养殖大鲵恶臭假单胞菌的分离鉴定及药物敏感性分析  

Isolation and Identificationof the Pseudomonas putida of Farmed from gian Salamander and Drug Sensitivity Analysis

作　　者：杨敏[1] 吴青 沙莎[1] YANG Min;WU Qing;SHA Sha(Department of Veterinary Medicine , Southwest University , Rongchang Campus , Chongqing 402460 , Chin)

机构地区：[1]西南大学荣昌校区动物医学系,重庆荣昌402460

出　　处：《中国兽医杂志》2018年第1期87-90,94,共5页Chinese Journal of Veterinary Medicine

基　　金：国家科技支撑计划项目(2012 BAD25 B10;2012BAD25B10-1);重庆开州区农委采购项目(2015ZFCG150)

摘　　要：为探究重庆某大鲵养殖基地大鲵死亡的原因,对从死亡大鲵的肾脏分离得到的菌株进行形态学观察,生物学特性分析,鲫鱼致病性试验,16S r DNA PCR鉴定,药敏试验和中药试验。结果显示,一株命名为KN-4株基因序列与恶臭假单胞菌(Pseudomonas putida)的同源性为98.9%,对鲫鱼有较强致病性。药物敏感性试验结果显示,KN-4菌株对头孢他啶、丁胺卡那霉素、新霉素、头孢曲松、多黏菌素B均敏感。中药试验结果表明,连翘、苦参、甘草、鱼腥草、黄连、黄芩、老鹤草对分离株KN-4有较强抑菌活性,而板蓝根、蒲公英等7种中药对其无抑菌作用。To identify the pathogenic bacteria from dead giant salamander（ Andrias davidianus） in a breeding base of Chongqing,we isolated some strains from the kidney samples of dead giant salamander. These isolates were identified by morphologic characters,biochemical identification,carp pathogenicity test,16 S rRNA sequence analysis,drug sensitive test and TCM test. The results showed that 16 S rRNA sequence of one of these isolates had high homology with the reference strains of Pseudomonas putida 98. 9%,and had high pathogenicity in craps. Susceptibility test showed that the strain was highly sensitive to ceftazidime; amikacin sulfate;neomycin; eftiaxone,and polymyxin. The traditional Chinese medicine test showed that forsythia suspensa,sophora flavescens,licorice,licorice,houttuynia cordata,coptis chinensis,scutellaria baicalensis and erodium stephanianum willd had stronger antibacterial activity for the KN-4 strain,but indigowoad root,dandelion and other seven kinds of traditional Chinese medicine had no antibacterial activity.

关 键 词：大鲵 恶臭假单胞菌 分离鉴定 药物敏感性分析 

分 类 号：S947.3[农业科学—水产养殖]