自然高低温胁迫下粳稻穗粒数相关性状稳定位点的发掘  

Mining Stable Locus at Natural High Temperature and Low Temperature Stress of Grain Number Related Traits in Japonica Rice

作　　者：江建华[1] 吴爽[1] 胡长敏[1] 景春雨 王德正[1] JIANG Jianhua;WU Shuang;HU Changmin;JING Chunyu;WANG Dezheng(Anhui Province Key Laboratory of Rice Genetics and Breeding/Hefei Branch of China National Center for Rice Improvement/ Institute of Rice Research,Anhni Academy of Agricultural Sciences,Hefei, Anhui 230031)

机构地区：[1]安徽省农业科学院水稻研究所/国家水稻改良中心合肥分中心/安徽省水稻遗传育种重点实验室,安徽合肥230031

出　　处：《核农学报》2018年第6期1070-1078,共9页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金项目(31601374);安徽省科技计划项目(1501031096);安徽省水稻遗传育种重点实验室绩效项目(2016SDSY004)

摘　　要：为发掘自然高低温胁迫下粳稻穗粒数3个相关性状的稳定位点,本研究对日本晴/越光//日本晴染色体片段置换系(CSSL)群体的48个株系在2015年庐江正常年份(E1)、2016年陵水自然低温(E2)以及2016年庐江自然高温(E3)3个不同温度环境下的每穗总粒数(GNP)、每穗实粒数(FGP)和结实率(SF)进行非条件QTL定位和将生育期矫正到相同水平下的条件QTL定位。结果表明,控制3个性状的QTL均以加性效应为主。CSSL群体中检测到控制每穗总粒数的稳定位点qGNP7.1,控制每穗实粒数的稳定位点qFGP6.1和qFGP11,控制结实率的稳定位点qSF9和qSF11。其中,qGNP7.1、qFGP11和qSF11的增效等位基因来自越光,qFGP6.1和qSF9增效等位基因来自日本晴。位于相同染色体片段的qFGP11和qSF11在3个环境中均能检测到,表明控制该位点的等位基因在自然高低温下均能稳定表达;qGNP7.1在E1和E2中均能检测到,表明该位点等位基因的表达不受自然低温的影响;qFGP6.1和qSF9均在E1和E3中检测到,表明以上2个位点等位基因的表达不受自然高温的影响。本研究结果为利用分子标记改良粳稻穗粒数3个相关性状及耐高低温新材料的选育提供了参考。To explore stable locus under natural high temperature and low temperature stress of grain number three related-traits in japonica. Unconditional QTL analysis and conditional QTL analysis by adjusted to an identical level of growth duration were conducted for grain number per panicle（GNP）,filled grain number per panicle（FGP） and percentage of spikelet fertility（SF） in a set of chromosome segment substitution lines（CSSLs） derived from a japonica cross of Nipponbare（recipient） and Koshihikari（donor）. The 48-line CSSL population was planted under normal temperature year of 2015 in Lujiang（E1）,low temperature year of 2016 in Lingshui（E2） and high temperature year of 2016 in Lujiang（E3）. Results showed that all QTLs for GNP,FGP and SF detected by the two methods were predominant additive effects. Stable loci of qGNP7. 1 of GNP,qFGP6. 1 and qFGP11 of FGP and qSF9 and qSF11 of SF were detected. Allele from Nipponbare can increase effect in qFGP6. 1 and qSF9. Allele from Koshihikari can increase effect in qGNP7. 1,qFGP11 and qSF11. qFGP11 and qSF11 on the same chromosome segment were detected in three environments. The result indicated that alleles of these loci could be stably expressed under natural high temperature and low temperature. qGNP7. 1 was detected in E1 and E2 showed that expression of allele from the locus was unaffected under low natural temperature. qFGP6. 1 and qSF9 were detected in E1 and E3 showed that expression of alleles from these two loci were unaffected at natural high temperature. These results will provide valuable reference information for improving grain number related-traits by molecular markers and breeding variety with natural high and low temperature stress.

关 键 词：粳稻 自然高低温胁迫 穗粒数 条件QTL定位 稳定位点 

分 类 号：S511.22[农业科学—作物学]