解淀粉芽孢杆菌Lx-11的促水稻生长作用及促生长物质分析  

Effect and Components Analysis of Cell Free Supernatant Produced by Bacillus amyloliquefaciens Strain Lx-11 on Rice Plant Growth-promoting

作　　者：张荣胜[1] 戴秀华[1,2] 刘永锋[1] 陈志谊[1] ZHANG Rongsheng;DAI Xiuhua;LIU Yongfeng;CHEN Zhiyi(Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing,Jiangsu 210014;Department of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu 210095)

机构地区：[1]江苏省农业科学院植物保护研究所,江苏南京210014 [2]南京农业大学植物保护学院,江苏南京210095

出　　处：《核农学报》2018年第6期1230-1238,共9页Journal of Nuclear Agricultural Sciences

基　　金：新型高效生物杀菌剂研发(2017YFD0201100);国家自然科学基金(31501691);江苏省农业科技自主创新资金CX(17)3021

摘　　要：生防菌Lx-11是一株对水稻细菌性条斑病具有较好防治效果的解淀粉芽孢杆菌,为明确其对水稻的促生作用,本研究以感病品种金刚30为试验材料,通过比较不同稀释倍数的生防菌Lx-11无菌上清滤液对水稻种子萌发和幼苗的生长的影响,测定其对水稻芽长、根长和株高,以及叶绿素a、叶绿素b和总叶绿素含量和根系活力的影响,运用液质联用仪检测无菌上清滤液中与促生相关的物质赤霉素、脱落酸、生长素和亚精胺的含量。结果表明,生防菌Lx-11无菌上清滤液稀释后对水稻种子和幼苗均有一定的促生作用,且随着菌液稀释倍数的增加,促生作用呈先增加后减少变化,稀释倍数为100倍时,对水稻种子和幼苗的形态和生理指标的影响最为显著;当稀释倍数达到200倍以上,其对水稻的促生效果下降。不同浓度无菌上清液处理水稻幼苗时,水稻植株的叶绿素a、叶绿素b、总叶绿素含量和根系活力均有不同程度的增加,其中以100倍稀释液增加最大,分别为45.97%、49.62%、46.95%和225.43%。生防菌Lx-11无菌上清滤液中生长素、赤霉素、脱落酸和亚精胺的含量分别为1 200、75.5、3 130和811ng·mL^(-1)。综上,生防菌Lx-11对水稻植株的生长具有明显的促进作用,且促生长物质主要存在于发酵上清液中。本研究为解淀粉芽孢杆菌Lx-11在微生物农药领域的田间高效应用提供了理论依据。Bacillus amyloliquefaciens Lx-11 is a biocontrol strain which has significant efficiency to control bacterial leaf streak of rice in the field trials. In this study,the sensitive variety Jiangang 30 was used as test material to compare the effects of different dilutions of the sterile supernatant of the Lx-11 strain on seed germination and growht of seedlings in rice. The effect of rice shoot length,root length,plant height,chlorophylls a,chlorophylls b,total chlorophylls and root activity of rice treated with cell free supernatant dilution of Lx-11 were investigated. The HPLC-MS methods were established for the determination of bioactive substances including auxin,abscisic acid,gibberellin and spermidine in suspension of Lx-11 strain filtrated with fiber filters. Results showed that different cell free supernatant dilutions of strain Lx-11 could promote seed germination and seedling growth of rice. The growth promoting effect was varied with the dilution times,with the dilution increased： the role of promoting growth was first increased and then decreased,in which the 100 times dilution was the most significant impact on the morphology and physiological indicators of rice seeds and seedlings,while the dilution of 200 times,the promoting effect reduced. The contents of chlorophyll in leaves and the root activity of plant were increased in different degrees after spraying the different dilutions. The highest was treated by the 100 times dilution,increased by 45. 97% in chlorophyll a,49. 62% in chlorophyll b,46. 95% in total chlorophyll and 225. 43% in root activity,respectively. The supernatant of Lx-11 containing IAA,GAs,ABA and Spermidine,were 1200 ng·mL^-1,75. 5 ng·mL^-1,3130 ng·mL^-1 and 811 ng·mL^-1 respectively,detected in the supernatant of strain Lx-11 by a liquid chromatography-mass spectrometry. In general, B. amyloliquefaciens strain Lx-11 could significantly improve the growth and development of rice plant,and the promotion substance presents mainly in the supernatant. This research provides a th

关 键 词：解淀粉芽孢杆菌 根系活力 促生长物质 液质联用仪 

分 类 号：S511[农业科学—作物学]