管灸对面神经损伤家兔面神经核内受体TrkC表达的影响  

Effects of Tube-moxibustion on the Expression of Receptor TrkC in the Nucleus of Facial Nerve for Rabbits with Facial Nerve Injury

作　　者：肖雪[1] 宋军[2] 鄢良春[2] 田丰伟 赵金发[1] 杨金蓉[1] XIAO Xue;SONG Jun;YAN Liangchun;et al(Chengdu University of Traditional Chinese Medicine Chengdu Sichuan 610072, China;Sichuan Provincial Academy of Traditional Chinese Medicine Chengdu Sichuan 610041, China)

机构地区：[1]成都中医药大学,四川成都610072 [2]四川省中医药科学院,四川成都610041 [3]重庆市中医研究院,重庆渝中400013

出　　处：《四川中医》2018年第3期59-62,共4页Journal of Sichuan of Traditional Chinese Medicine

基　　金：国家自然科学基金课题(编号:81373740)

摘　　要：目的:研究管灸对面神经损伤家兔面神经核中受体Trk C表达的影响。方法:采用机械压榨法制作家兔面神经损伤模型,将家兔随机分为假手术组、模型组、管灸组、热疗组,通过免疫组化学、组织原位杂交和RT-PCR三种方法分别检测各组面神经核中受体Trk C蛋白和基因的表达变化。结果:在三种方法检测下发现家兔面神经损伤后面神经核Trk C的表达均明显下降(P<0.01),管灸组面神经核Trk C的表达均显著高于模型组(P<0.01)结论:管灸疗法可显著提高面神经核中受体Trk C的表达,这可能对促进面神经损伤后的再生和修复有重要作用。Objective： To study the effect of tube-moxibustion on the expression of receptor tyrosine kinase C in the facial nucleus of rabbits whose facial nerve were injured. Methods： The model of facial nerve injury in rabbits was made by mechanical press and the rabbits were randomly divided into sham operation group,model group,tube-moxibustion group and thermotherapy group. The expression of Trk C protein levels and Trk CmRNA were analyzed by immunohistochemistry, in situ hybridization（ ISH） and reverse transcriptase-polymerase chain reaction（ RT-PCR）. Results： Detected by the three methods found that the expression of Trk C in facial nucleus following rabbit facial nerve injury was evidently decreased（ P 0. 01）,however,in the tube-moxibustion group it was significantly increased than that in the model group（ P 0. 01）. Conclusions： Tube-moxibustion can significantly increase the expression of Trk C in the facial nucleus,which may play an important role in promoting the regeneration and repair of facial nerve injury.

关 键 词：管灸 面神经核 TRKC 面神经再生 

分 类 号：R245[医药卫生—针灸推拿学]