HPLC法测定乳汁中奥硝唑浓度  

Determination of ornidazole concentration in breast milk by HPLC

作　　者：叶朝辉[1] 余莉[2] 苗彩云[1] 陆晓东 毛瑛瑛 YE Chaohui;YU Li;MIAO Caiyun;LU Xiaodong;MAO Yingying(Department of Pharmacy, Ningbo Women ＆ Children Hospital of Zhefiang Province ,Ningbo 315012, China;Department of Pharmacy, The Affiliated Hospital of School of Medicine of Ningbo University, Ningbo 315020, China)

机构地区：[1]浙江省宁波市妇女儿童医院药剂科,宁波315012 [2]宁波大学医学院附属医院药剂科,宁波315020

出　　处：《中国临床药学杂志》2018年第2期99-101,共3页Chinese Journal of Clinical Pharmacy

基　　金：宁波市医学科技计划项目(编号2014A09)

摘　　要：目的建立HPLC法测定乳汁中奥硝唑的浓度,并监测产后用药妇女乳汁中奥硝唑含量。方法采用Agilent Eclipse XDB-C18柱(150 mm×4.6 mm,5μm),以甲醇-水(27.5∶72.5)为流动相,甲硝唑为内标,检测波长320 nm,流速1.0m L·min-1,柱温30℃。收集产后使用奥硝唑的妇女乳汁,进行乳药浓度监测。结果乳汁中奥硝唑质量浓度在0.875~54.95 mg·L^(-1)内线性关系良好,r=0.999 3,乳汁中最低检测限为0.075 mg·L^(-1)。在低、中、高3种浓度下,平均加样回收率分别为98.54%、101.34%和103.11%。对9位产后用药妇女乳汁中奥硝唑含量监测结果为1.88~10.86 mg·L^(-1)。结论本研究建立的HPLC法可快速、准确地用于测定乳汁中奥硝唑的浓度。产后用药妇女乳汁中奥硝唑含量较高、且个体差异较大,建议在连续用药期间暂停授乳。AIM To establish an HPLC method for determination of ornidazole concentration in breast milk and monitor the ornidazole milk concentration after delivery. METHODS The drug was detected by UV detector at wavelength of 320 nm with a Agilent Eclipse XDB-Cls column （150 mm×4.6 mm, 5 μm）. The mixture of methanol and water （27.5 ： 72.5 ） was used as the mobile phase and metronidazole was used as the internal stand- ard. The flow rate was 1.0 mL·min^-1 and the column temperature was 30 ℃. The breast milk in parturient was collected to monitor ornidazole milk concentration. RESULTS The calibration curve was linear over the range of 0. 875 - 54.95 mg - L- 1 with good correlation coefficient （ r = 0. 999 3 ） and the measurable limit of detection was 0. 075 mg·L^-1. The average sample recoveries were 98.54% ,101.34% and 103.11% for low, middle and high concentration of quality control, respectively. The monitoring results of the concentration of omidazole in the milk of 9 postpartum women were 1.88 - 10.86 mg·L^-1. CONCLUSION This HPLC method of detecting ornidazole milk concentration is quick and precise. The ornidazole milk concentration in parturient has a high level during continuous administration, and milk lactation is not recommended.

关 键 词：奥硝唑 HPLC 乳药浓度 

分 类 号：O657.72[理学—分析化学] R969[理学—化学]