标本配穴电针预处理对慢性心肌缺血模型大鼠心肌线粒体呼吸功能的影响  

Effects of Electroacupuncture Preconditioning at Biao-Ben Acupoints Compatibility on Myocardial Mitochondrial Function in Rats with Chronic Myocardial Ischemia Model

作　　者：陈松 陈茜[2] 吴松 王华 梁凤霞 黄伟[3,4] CHEN Song;CHEN Qian;WU Song;WANG Hua;LIANG Fengxia;HUANG Wei(College of Acupuncture -Moxibustion and Orthpaedics, Hubei University of TCM, Hubei Collaborative Innovation Center, Acupuncture and Moxibustion for Disease Prevention, Wuhan 430061, Hubei,China;Doctoral Candidate in 2017,College of Acupuncture - Moxibustion and Orthpaedics, Hubei University of TCM, Wuhan 430061, Hubei, China;Clinical Medical College, Hubei University of TCM, Wuhan 430061, Hubei, China;Hubei Hospital of TCM, Wuhan 430061, Hubei, China)

机构地区：[1]湖北中医药大学针灸骨伤学院针灸治未病湖北省协同创新中心,湖北武汉430061 [2]湖北中医药大学针骨学院2017级博士研究生,湖北武汉430061 [3]湖北中医药大学临床医学院,湖北武汉430061 [4]湖北省中医院,湖北武汉430061

出　　处：《辽宁中医杂志》2018年第4期828-831,I0002,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81273865,81473789);湖北中医药大学2017年度“青苗计划”资助项目(2017ZZX024)

摘　　要：目的：探讨标本配穴电针预处理对慢性心肌缺血损伤过程中心肌线粒体的保护作用。方法：本实验选用3月龄Wistar雄性大鼠45只,体质量180~220 g,编号后按随机数字表分入空白组、模型组、标本配穴电针预处理组,每组15只。模型组大鼠连续7天在颈背部皮肤丰厚处按照5 mg/kg行皮下注射盐酸异丙肾上腺素建造慢性心肌缺血模型,观察线粒体的呼吸功能控制比和能量等指标及形态变化。结果：模型组大鼠部分心肌肌丝溶解、断裂,心肌细胞轻微肿胀,轻度空化,线粒体排列稍紊乱,部分线粒体水肿、存在空泡样病变。标本配穴电针预处理组的大鼠线粒体RCR处于正常水平,明显高于模型组,存在显著性差异（P〈0.01）,但标本配穴电针预处理组线粒体RCR低于空白组,差异显著（P〈0.01）。结论：标本配穴电针预处理能有效保护心肌线粒体的功能和结构,防止慢性心肌缺血中心肌组织的进一步破坏。Objective： To explore the protective effects of electroacupuncture preconditioning at Biao-Ben acupoints compatibility on myocardial cells in the process of chronic myocardial ischemia model. Methods： In this experiment,45 male Wistar rats,3-month-old,weighting about 180-220 g,were randomly divided into three groups： no-treatment control group（ NC group）,chronic myocardial ischemia model group（ M group） and electroacupuncture preconditioning group（ EA group）,with 15 rats in each group respectively. Chronic myocardial ischemia model was achieved by the received injection of isopropylarterenol hydrochloride,5 mg/kg per day,for 7 days. Before establishing model,the acupuncture group was treated with electric acupuncture for20 min,1 m A,continuous wave,for 7 days. Then myocardial tissue were taken out and changes of the respiratory control ratio of mitochondrial,the content of ATP and the ultrastructures were observed. Results： In the EA group,the structure of cardiac tissue was normal except for a few mild edemata among cells,interstitial and mitochondria. The RCR of mitochondria in the EA group was in the normal level and significantly higher than that in the M group（ P 〈 0. 01）,but lower than that in the NC group（ P 〈0. 01）. Conclusion： Electroacupuncture preconditioning at Biao-Ben acupoints compatibility could repair the damage causing by chronic myocardial ischemia in myocardial tissue and plays a positive role in the protection of structure and function of mitochondria.

关 键 词：标本配穴 预处理 慢性心肌缺血 线粒体 

分 类 号：R542.2[医药卫生—心血管疾病]