消癌舒对小鼠宫颈癌U14移植瘤的抑制作用  

Inhibitory Effect of Xiaoaishu(XAS) on Cervical Carcinoma U14 Xenografts in Mice

作　　者：金海[1] 龙洪相 陈积[3] 吴芹[2] 聂晶[2] 王井洪 金凤[2] JIN Hai;LONG Hongxiang;CHEN Ji;WU Qin;NIE Jing;WANG Jinghong;JIN Feng(Zunyi Medical University ： a. Institute of Digestive Diseases of Attached Hospital, b. Key Laboratory of Basic Pharmacology of Ministry of Education, c. College of Pharmacy, Zunyi 563000, Guizhou, China;Guizhou Jinghong Biotechnology Limited Liability Company, Liuzhi 553400, Guizhou, China)

机构地区：[1]遵义医学院附属医院消化病研究所,贵州遵义563000 [2]遵义医学院基础药理省部共建教育部重点实验室,贵州遵义563000 [3]遵义医学院药学院,贵州遵义563000 [4]贵州景红生物科技发展有限责任公司,贵州六枝553400

出　　处：《辽宁中医杂志》2018年第4期835-838,I0003,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：贵州省科技厅中药现代化专项项目(黔科合中药字20115008号);贵州省教育厅自然科学类科研项目(黔教科2011056号);贵州省中医药管理局项目(D274,QZYY2010-59);国家自然科学基金项目(81360311,81660599)

摘　　要：目的：观察消癌舒对小鼠宫颈癌细胞株U14移植瘤的抑制作用。方法：U14瘤株接种C57小鼠腹腔传代,传第三代时取腹水接种昆明种小鼠腋窝皮下。将小鼠随机分为模型组、顺铂组、消癌舒低、中、高剂量组及联合用药组。接种次日开始给药。消癌舒低、中、高剂量组分别给予消癌舒0.75、1.5、3.0 g·kg-1每日灌胃给药1次;顺铂组腹腔注射顺铂3 mg·kg-1隔日1次,联合用药组给予消癌舒0.75 g·kg-1加顺铂3 mg·kg-1,消癌舒及顺铂给药同上,模型组给予等体积的蒸馏水。连续给药14 d。给药后每3天测量小鼠移植瘤体积并绘制肿瘤生长曲线;给药结束后次日处死小鼠,剥离瘤体,称瘤重并计算抑瘤率;记录用药期间各组小鼠的体重,并绘制体重变化曲线。结果：消癌舒0.75 g·kg-1组小鼠瘤体积及瘤重与模型组比较无明显变化（P〉0.05）,抑瘤率仅为14.12%,而消癌舒1.5、3.0 g·kg-1组小鼠的肿瘤体积及瘤重均明显低于模型组（P〈0.05）,抑瘤率分别为42.05%、61.40%,消癌舒各剂量组小鼠体重与模型组比较均无明显变化（P〉0.05）;顺铂组及联合用药组小鼠的瘤体积及瘤重均明显低于模型组（P〈0.05）,抑瘤率分别为90.56%、96.36%,而联合用药组小鼠的瘤重明显低于顺铂组（P〈0.05）;顺铂组及联合用药组小鼠体重均明显低于模型组（P〈0.05）,但顺铂组小鼠体重下降比联合用药组更明显,差异有统计学意义（P〈0.05）。结论：消癌舒对小鼠宫颈癌细胞株U14移植瘤具有抑制作用,与顺铂联用效果更显著,并能减轻顺铂的毒性作用。Objective： To observe the inhibitory effect of Xiaoaishu（ XAS） on cervical carcinoma U14 xenografts in mice.Methods： U14 tumor strain was inoculated by intraperitoneal injection in C57 mice and passaged to the third generation,and then ascites were extracted and inoculated subcutaneously in the armpit of KM mice. The mice were randomly divided into model group,cisplatin group（ 3 mg·kg-1）,XAS groups（ 0. 75 g·kg-1,1. 5 g·kg-1,3 g·kg-1） and combination group（ XAS 0. 75 g·kg-1＋ cisplatin 3 mg·kg-1）. All drugs were given in the next day after inoculation. XAS was given orally once a day for 14 days. Cisplatin was given through intraperitoneal injection once every other day. Model group was given the equal volume of distilled water. The tumor volume was measured in every 3 days and the growth curve was drawed. The mice were killed in the next day after administration. The xenografts were extracted and weighed,which inhibition rate was calculated. The body weight of mice during the administration period was recorded and the weight change curve was drawed. Results： XAS 0. 75 g·kg-1 had no inhibitory effect on tumor（ P 〉 0. 05） and the inhibitory rate was only 14. 12%. While the volume and weight of tumor in 1. 5 g ·kg-1,3. 0 g·kg-1 groups were significantly decreased compared with those of the model group（ P 〈 0. 05）,and the tumor inhibition rates were 42. 05% and 61. 40% respectively. The body weights in different XAS groups had no obvious change compared with those of the model group. The volume and weights of tumor in cisplatin and combination groups were significantly lower than those of the model group,and the tumor inhibition rates were 90. 56% and 96. 36% respectively. The tumor weights in combination group were significantly lower than those of the cisplatin group（ P 〈 0. 05）. The body weights in cisplatin and combination groups were significantly decreased compared with those of the model group（ P 〈 0. 05）,but the body weight in cisplatin group was signifi

关 键 词：消癌舒 宫颈癌 小鼠 U14 

分 类 号：R285.5[医药卫生—中药学]