小半夏汤对化疗大鼠胃肠黏膜修复及其抗氧化应激作用的研究  

Effects of Xiaobanxia Decoction Attenuating Cisplatin-induced Gastrointestinal Mucositis Rats and Its Oxidative Stress

作　　者：杜静[1] 张启龙[1] 李贵生[1] 聂克 DU Jing;ZHANG Qilong;LI Guisheng;NIE Ke(Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China;Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong, China)

机构地区：[1]山东中医药大学,山东济南250355 [2]广州药科大学,广东广州510006

出　　处：《辽宁中医杂志》2018年第4期851-853,I0004,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：山东省高等学校科技计划项目(J16LK07);国家自然科学基金面上项目(81373828,81673779)

摘　　要：目的：通过观察小半夏汤对化疗致胃肠道黏膜炎模型大鼠胃、回肠组织形态学的改变及其对抗氧化应激标志物的影响,探讨小半夏汤防治化疗致胃肠道黏膜炎的作用机制。方法：将112只Wistar大鼠随机分为7组,采用顺铂建立胃肠道黏膜炎模型,动物分别于24 h和72 h两个时间点取材、处死,HE染色观察胃和回肠组织病理学改变,检测血清中ROS含量,以及SOD和GSH-Px的活性。结果：小半夏汤各剂量治疗组可以改善化疗大鼠胃肠道黏膜损伤,与模型组比较,小半夏汤各剂量治疗组可以使化疗大鼠ROS含量明显降低（P〈0.05）,SOD和GSH-Px活性明显升高（P〈0.05）。结论：小半夏汤可以改善大鼠化疗致胃肠道黏膜损伤,可能与其抗氧化应激作用有关,具体机制还需进一步阐明。Objective： To observe effects of Xiaobanxia Decoction on cisplatin-induced gastrointestinal mucositis rats and its oxidative stress and explore the mechanism of its treatment and prevention. Methods： Totally 112 rats were randomly selected as 7 groups. At 24 h and 72 h after model establishment,the rats were sacrificed and the blood,antrum and ileum of all rats were extracted. Pathological histology of tissue was observed with HE staining and serum ROS,SOD and GSH-Px were checked. Results： Xiaobanxia Decoction can abate the pathological changes of autrum and ileum tissues of the model. Compared with the model group,serum ROS was reduced and SOD and GSH-Px activities were increased to varying degrees in each treatment group. Conclusion：The conventional dosage of Xiaobanxia Decoction can attenuate cisplatin-induced gastrointestinal mucositis. It might be related to the inhibitory effect on oxidative stress and some questions need further discussion.

关 键 词：小半夏汤 化疗 胃肠道黏膜炎 氧化应激 大鼠 

分 类 号：R285.5[医药卫生—中药学]