芪参益气滴丸预适应对缺氧复氧损伤心肌细胞的延迟保护作用及与HSP70的关系  

Delayed Protection of QiShen YiQi Dripping Pills-induced Preconditioning to Hypoxia/reoxygenation Injury of Myocardial Cells and Its Connections with HSP70

作　　者：马妍 郭利平[2] 樊官伟[3] 任明[1] 张磊[3] 王怡[2] 李岩 孙晓 赵宏杰[2] 刘丹[4] MA Yan;GUO Liping;FAN Guanwei;REN Ming;ZHANG Lei;WANG Yi;LI Yan;SUN Xiao;ZHAO Hongjie;LIU Dan(Baokang Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin University of Traditional Chinese Medicin;First Teaching Hospital of Tianjin University of TCM;Wuqing District TCM Hospital of Tianjin Municipality)

机构地区：[1]天津中医药大学附属保康医院,天津300193 [2]天津中医药大学 [3]天津中医药大学第一附属医院 [4]天津市武清区中医医院

出　　处：《西部中医药》2018年第4期10-14,共5页Western Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(编号81072965);国家自然科学基金面上项目(编号81273993);高等学校全国优秀博士论文作者专项基金(编号200779)

摘　　要：目的:研究芪参益气滴丸预适应对缺氧复氧损伤心肌细胞的延迟保护作用,并进一步探讨其作用机制及与热休克蛋白70(HSP70)表达的关系。方法:建立心肌细胞缺氧复氧损伤(H/R)及缺氧预适应(HPC)模型,以芪参益气滴丸预适应(QS)与HPC分别或同时作用于心肌细胞,并加入N-乙酰半胱氨酸(NAC)作为抑制剂取消HPC的延迟保护(DP)效应,在培养24小时后对心肌细胞进行H/R损伤,通过镜下观察细胞形态,检测CCK-8、乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)以及HSP70的蛋白含量和m RNA表达,观察心肌细胞的损伤程度及HSP70的变化。结果:在经过H/R损伤后,QS组与HPC组心肌细胞表现出很好的细胞活性与抗氧化力,损伤轻(P<0.05),HSP70蛋白含量和m RNA表达均高于H/R组(P<0.5);NAC抑制了HPC对心肌细胞的延迟保护作用,表现为细胞活性降低、抗氧化能力减弱,损伤较重(P<0.05),HSP70蛋白含量及m RNA表达显著降低(P<0.05);HPC+NAC+QS组心肌细胞表现出很好的活性与抗氧化能力,损伤轻(P<0.05),HSP70蛋白含量和m RNA表达显著升高(P<0.5)。结论:芪参益气滴丸预适应对缺氧复氧损伤心肌细胞具有延迟保护作用,其作用效果与缺氧预适应相当,其作用机制与多途径上调HSP70的表达有关。Objective： To discuss delayed protection of Qi Shen Yi Qi dripping pills（QSYQ）-induced preconditioning to hypoxia/reoxygenation injury of myocardial cells, and to further discuss the connections between its mechanism and heat shock protein 70（HSP70） expression. Methods： After establishing mycardial cells of hypoxia/reoxygenation injury（H/R） and hypoxia preconditioning（HPC）, Qi Shen Yi Qi dripping pills preconditioning and HPC were used to act on myocardial cells respectively or simultaneously, and N-acetyl-L-cysteine（NAC） was added as the inhibitor to cancel delayed protection effects of HPC, H/R injury was given to myocardial cells after they were cultivated for 24 hours, HSP70 protein contents and m RNA expressions, SOD, MDA, LDH and CCK-8 were detected by microscopic observation of cellular morphology, the changes of HSP70 and the injury of myocardial cells were observed. Results：After experiencing H/R injury, myocardial cells in QS group and HPC group performed the best cell activity and anti-oxidation with light injury（P〈0.05）, HSP70 protein contents and m RNA expressions were higher than these of H/R group（P〈0.05）; NAC inhibited the delayed protection of HPC to myocardial cells, which performed low cell activity, anti-oxidation ability weakening and severe injury（P〈0.05）, HSP70 protein contents and m RNA expression lowered notably（P〈0.05）; myocardial cells in HPC＋NAC＋QS group showed better cell activity and anti-oxidation with light injury（P〈 0.05）, HSP70 protein contents and m RNA expression lifted notably（P〈 0.05）. Conclusion：Qi Shen Yi Qi dripping pills preconditioning shows delayed protection to myocardial cells with H/R injury, and its effects are equivalent to these of HPC, and its mechanism might be related to up regulating HSP70 expressions by many ways.

关 键 词：芪参益气滴丸 预适应 心肌细胞 HSP70 延迟保护 

分 类 号：R542.22[医药卫生—心血管疾病]