苦瓜总皂苷对盐敏感高血压致肾脏损害大鼠肾脏组织中内皮素-1表达的影响  

Effect of bitter gourd saponins on the expression of ET-1 in renal tissues of rats with renal damage induced by salt-sensitive hypertension

作　　者：赵世莉 张云芳[1] 王琦[1] 雷莹 化宝军[1] Zhao Shili;Zhang Yunfang;Wang Qi;Lei Ying;Hua Baojun(Department of Nephrology , the People＇s Hospital of Huadu District, Guangzhou, Guangdong 510800, China)

机构地区：[1]广州市花都区人民医院肾病科,广东省广州510800

出　　处：《中国基层医药》2018年第4期423-428,J0001,共7页Chinese Journal of Primary Medicine and Pharmacy

基　　金：广东省广州市花都区科技工业和信息化局项目（15-HDWS-016、16-HDWS-32）

摘　　要：目的 探讨苦瓜总皂苷对盐敏感高血压所致肾脏损害大鼠是否有治疗作用,并分析其可能的作用机制.方法 50只SD大鼠在适应性喂养10 d后采用随机数字表法分为五组,分别为正常对照组、模型对照组以及苦瓜总皂苷低、中和高剂量治疗组,每组10只,采用高盐饲料诱导盐敏感高血压肾脏损害大鼠模型,造模成功后给予相应治疗（正常组、模型组0.9％氯化钠溶液1 mL·kg-1·d-1灌胃,苦瓜总皂苷三个剂量组分别给予苦瓜总皂苷10、20和40 mg·kg-1·d-1灌胃）,8周后处死大鼠,HE染色检测其肾脏病理学,同时比较分析各组血压、心率、尿量和肾功能的变化特点,最后免疫组化、Western Blotting以及半定量PCR检测肾脏组织中内皮素-1（ET-1）的变化特点.结果 大鼠收缩压比较,在整个治疗过程中,正常组收缩压约120 mmHg,而模型组收缩压约170 mmHg,苦瓜总皂苷三个剂量组相较于模型组在治疗第2周开始就明显低于模型组（P〈0.05）（低、中和高剂量组t值为1.765、1.982和2.126,均P〈0.05）,之后进一步改善;心率在整个治疗期间内五组差异无统计学意义（均P〉0.05）;与正常组相比,模型组尿素氮（BUN）、肌酐（Cr）、尿酸（UA）、尿量、尿蛋白和N-乙酰-β-D-葡萄糖苷酶（NAG）水平明显升高（t值依次为28.703、33.932、29.298、4.695、10.989和10.871,均P〈0.05）,而苦瓜总皂苷相较于模型组均明显降低（均P〈0.05）,随着苦瓜总皂苷剂量的明显增加,降低的趋势更加明显;HE染色明显看出,正常组大鼠切片完全正常,模型组有明显肾小球硬化和肾间质纤维化,苦瓜总皂苷治疗后,三个治疗组均可以明显改善;各组大鼠ET-1免疫组化、western blotting和半定量PCR可见,正常组ET-1表达较少,与正常组相比,模型组表达明显升高（蛋白t值为14.650、mRNA的t值为11.387,均P〈0.05）,苦瓜总皂苷三个治疗组明显降低.结论 苦瓜总皂苷可明显改Objective To investigate the effect of bitter gourd saponins on the salt -sensitive hypertension caused kidney damage in rats ,and analyze the mechanism of its therapeutic effect .Methods 50 SD rats were fed for 10 days with normal diet ,and then based on random number method ,the rats were randomly divided into 5 groups：the normal control group,model control group and the low total saponins bitter,medium and high dose treatment group,10 rats in each group.Then,the rats in the normal control group and the model control group were given 1mL· kg-1 · d-1 normal saline,and the rats in bitter gourd saponins groups were given high salt diet 8 weeks to establish the model .After 4 weeks,the total saponins of bitter gourd treatment groups were given 10,20 and 40mg· kg-1· d-1.8 weeks later, the rats were sacrificed and the renal pathology was detected by HE staining .The changes of blood pressure , heart rate,urinary function and blood renal function were also analyzed .At last,Western blot and semi -quantitative PCR were used to detect the endothelin -1 （ET-1） in renal tissue.Results For the systolic blood pressure,in whole of treatment,the normal control group maintained at about 120 mmHg, while the model control group maintained at 170mmHg,which of the bitter gourd saponins three doses groups compared to the model control group was significantly lower （t=1.765,1.982,2.126,all P〈0.05）,further improved.And the heart rate had no statistically significant difference among the groups（all P〉0.05）.Compared with the normal control group,the blood urea nitrogen （BUN）, creatinine （Cr）,uric acid （UA）,urine volume,urinary protein and N -acetyl beta -D-Glucosaminidase （NAG） levels in the model control group were significantly increased （t=28.703,33.932,29.298,4.695,10.989,10.871, all P〈0.05）,which in the bitter gourd saponins treatment groups were significantly decreased （all P〈0.05）.HE staining showed that the rats in the normal group were normal ,and the model control group had ob

关 键 词：高血压 苦瓜总皂苷 肾机能不全 内皮素-1 

分 类 号：R285.5[医药卫生—中药学]