乙苯对CPCs细胞损伤及线粒体膜电位的影响  

Effects of ethylbenzene on cell injury and mitochondrial membrane potential of CPCs

作　　者：孙靓[1] 张明[2] 徐鹏[1] 樊琳[1] 崔博 曾强[2] 顾清[2] Sun Liang;Zhang Ming;Xu Peng;Fan Lin;Cui Bo;Zeng Qiang;Gu Qing(The Public Health of Tianjin Medical University, Tianjin 300070, Chin)

机构地区：[1]天津医科大学公共卫生学院,300070 [2]天津市疾病预防控制中心 [3]军事科学院军事医学研究院环境医学与作业医学研究所

出　　处：《中华劳动卫生职业病杂志》2018年第2期146-149,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金面上项目（81573123）;天津市自然科学基金青年项目（15JCQNJC45100）;天津市卫生局基金项目（2014KR13）

摘　　要：目的探讨乙苯对耳蜗前体细胞（CPCs）生长形态、增殖能力及线粒体膜电位（MMP）的影响，为乙苯导致听力损失的机制研究奠定基础。方法分离SPF级SD新生大鼠原代CPCs，荧光显微镜鉴定分离培养成功后，分为对照组和染毒组经不同浓度（0、15、30、45 μmol/L）的乙苯染毒24 h。采用倒置光学显微镜观察细胞损伤的形态学变化，MTT比色法检测细胞的增殖能力，荧光探针JC-1测定MMP的变化。结果大鼠CPCs的鉴定结果显示，肌球蛋白（Myosin）VIIa和内吞作用辅助蛋白（Epsin）表达阳性；与对照组相比，各染毒组大鼠CPCs的折光度增强，细胞死亡数目增多；与对照组相比，各染毒组的CPCs增殖能力明显降低并呈一定的剂量-效应关系，差异均有统计学意义（P〈0.05）；与对照组相比，各染毒组大鼠MMP明显降低，差异均有统计学意义（P〈0.05）。结论乙苯可能导致大鼠CPCs发生损伤，细胞增殖抑制及MMP降低。ObjectiveTo investigate the effects of ethylbenzene on growth morphology、proliferation ability and mitochondrial membrane potential（MMP）of cochlear progenitor cells（CPCs）,and to lay a foundation for the mechanism of hearing loss induced by ethylbenzene.MethodsWe can use the fluorescence microscopy to identify the original CPCs isolated from the newborn rats,and followed by the addition of different concentrations of ethylbenzene（0,15,30,45 μmol/L）for 24 hours.The morphological changes of cell injury were observed by inverted optical microscope.The proliferation ability of cells was detected by MTT colorimetry,and the change of MMP was detected by fluorescent probe JC-1.ResultsThe results of CPCs identification showed the expression of Myosin VIIa and Epsin positive;The results observed by inverted optical microscope showed all groups of CPCs morphological changes compared with the control group;MTT results showed that the decreased significantly proliferation ability of CPCs groups compared with the control group and a dose effect relationship with statistically significant difference（P〈0.05）;JC-1 test results showed the decreased significantly mitochondrial membrane potential in the treated group compared with the control group,and there was a statistically significant difference（P〈0.05）.ConclusionEthylbenzene may cause damage to CPCs,inhibition of cell proliferation and decrease of MMP in rats.

关 键 词：乙苯 大鼠耳蜗前体细胞 细胞损伤 线粒体膜电位 

分 类 号：R135[医药卫生—劳动卫生]