赤眼鳟TRIF基因cDNA克隆及其组织表达分析  

cDNA Cloning and Tissue Expression Analysis of TRIF in Squaliobarbus Curriculus

作　　者：徐莹[1] 李耀国[1,2] 王荣华 刘巧林[1,2] 王静安[1] 赵鑫 肖调义[1,2] XU Ying;LI Yao-guo;WANG Rong-hua;LIU Qiao-lin;WANG Jing-an;ZHAO Xin;XIAO Tiao-yi(Hunan Engineering Research Center for Utilization of Characteristics of Aquatic Resources, Hunan Agricultural University, Changsha 410128, China;Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde 415000, China)

机构地区：[1]湖南农业大学湖南省特色水产资源利用工程技术研究中心,中国长沙410128 [2]水产高效健康生产湖南省协同创新中心,中国常德415000

出　　处：《湖南师范大学自然科学学报》2018年第2期26-33,共8页Journal of Natural Science of Hunan Normal University

基　　金：国家自然科学基金资助项目(31272652); 中国博士后科学基金资助项目(2017M612560)

摘　　要：为探究诱导β干扰素包含TIR结构域的接头分子(TIR-domain-containing adaptor inducing interferon-β,TRIF)是否参与赤眼鳟(Squaliobarbus curriculus)抗病免疫反应,克隆获得了2 263 bp的赤眼鳟TRIF基因(Sc TRIF)全长c DNA.研究结果显示Sc TRIF具有TIR结构域,Sc TRIF和同属亚罗鱼亚科的草鱼TRIF聚为一支.该基因在赤眼鳟12个组织中均有表达,其中鳃中表达水平最高.感染草鱼呼肠弧病毒后,头肾和脾脏组织中Sc TRIF表达水平均呈现先下降后上调的趋势.研究表明Sc TRIF参与赤眼鳟抗GCRV病毒的免疫应答反应.In order to investigate the potential role of TIR-domain-containing adaptor induced interferon-β receptor of Squaliobarbus curriculus（ Sc TRIF） that plays in anti-disease immune reaction,the full c DNA sequence of Sc TRIF（ 2 263 bp） was cloned. Our research results in this paper showed that Sc TRIF protein possesseda typical structure of TIR domain,and Sc TRIF was clustered together with that of Ctenopharyngodon Idella,belonging to the subfamily of Leuciscinae. The expression of Sc TRIF was detected in 12 tissues,with the highest expression level in gill. After GCRV infection,the Sc TRIF expression level showed an overall tendency of firstly down-regulated and then increased,in both the head kidney and spleen tissues. Our results indicate that Sc TRIF involved in the immune response of Squaliobarbus curriculus against GCRV.

关 键 词：赤眼鳟 TRIF基因 CDNA 组织 GCRV 

分 类 号：Q785[生物学—分子生物学] S965[农业科学—水产养殖]