叶面喷施褪黑素调控水稻幼苗耐盐性的浓度效应研究  

Concentration-Dependent Effect of Foliar Spraying of Melatonin on Salt Tolerance of Rice

作　　者：宋雪飞 甘淳丹 赵海燕[2] 孙志国[2,3] 李刚华 张春明[2,3,5] 陈刚 郑青松[2,3] SONG Xuefei;GAN Chundan;ZHAO Haiyan;SUN Zhiguo;LI Ganghua;ZHANG Chunming;CHEN Gang;ZHENG Qingsong(College of Biological Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China;College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, China;Jiangsu Provincial Key Laboratory of Marine Biology, Nanjing 210095, China;College of Agronomy, Nanjing Agricultural University, Nanjing 210095, China;College of Grassland and Environmental Science, Xinjiang Agricultural University, Urumqi 830052, China)

机构地区：[1]扬州大学生物科学与技术学院,江苏扬州225009 [2]南京农业大学资源与环境科学学院,南京210095 [3]江苏省海洋生物学重点实验室,南京210095 [4]南京农业大学农学院,南京210095 [5]新疆农业大学草业与环境科学学院,乌鲁木齐830052

出　　处：《土壤学报》2018年第2期455-466,共12页Acta Pedologica Sinica

基　　金：国家科技支撑计划项目(2015BAD01B03;2015BAD01B01);江苏省重点研发计划(现代农业)重点项目(BE2015337-3)资助~~

摘　　要：为了探讨褪黑素(MT)对植物耐盐性的调控,以水稻"盐稻12号"为试验材料,研究了外源MT对75 mmol L-1 Na Cl胁迫下水稻幼苗株高、干重(DW)、根冠比(R/S)、氮磷钾(NPK)及钠(Na)含量的影响,并计算不同处理下各器官的K/Na和K、Na的选择性比率(SK,N a)。结果表明:盐胁迫下,外源喷施25~400μmol L-1 MT能够有效提高水稻植株的株高、DW,降低R/S;且随着MT浓度的升高,该效应越发显著,在MT施用浓度为200μmol L-1时,植株株高和DW均达到最大值。MT浓度为200和400μmol L-1时,植株生长指标差异均不显著。盐胁迫下,喷施MT明显提高植株的NPK含量,降低Na含量,显著增加氮转运系数(N-TF)、磷转运系数(P-TF)和钾转运系数(KTF),显著提高植株根吸收SK,Na(ASK,N a),而叶片运输SK,Na(TSK,Na)随着MT浓度增加逐渐降低。综上所述,盐胁迫下,喷施25~400μmol L-1 MT可明显提高水稻幼苗的NPK吸收,降低植株Na的积累,显著提高了水稻幼苗对K的选择性吸收,维持体内的离子稳态,增加植株地上部和根部生物量积累,从而显著提高水稻的耐盐性,其中,提高水稻耐盐的最适MT浓度为200μmol L-1。【Objective】 Soil salinization is a soil problem getting more and more serious nowadays, reducing plant growth and affecting agricultural production. The area of salinized rice fields is expanding, what is more, rice（Oryza sativa L.）, a main staple food crop of the country is quite sensitive to salt stress. More and more attention has been paid to the study on how to regulate salt tolerance of rice. Melatonin（MT） is an important class of indole compounds widely found in most organisms. More and more studies have found that though very low in content in plants, MT plays a very important role in physiological regulation and enhancement of plants. However, little has been found in literature on application of melatonin to rice. It is, therefore, of great significance to explore concentration-dependent effects of MT on salt tolerance of rice and its physiological mechanism. 【Method】A hydroponics experiment was carried out on effects of foliage spraying of MT to rice on plant height, biomass, root/shoot ratio, absorption and transportation of N, P, K and Na, K/Na ratio and SK,Na（K/Na selectivity ratio）. In this study, pass boxes were used for hydroponic cultivation of rice and extraneous MT sprayed. The experiment was designed to have 7 treatments, i.e.（1） Nutrient solution ＋ spraying deionized water（Control）;（2） Nutrient solution ＋ 75 mmol L^-1 Na Cl ＋ spraying deionized water（S75）;（3） Nutrient solution ＋ 75 mmol L^-1 Na Cl ＋ spraying 25 μmol L^-1 MT（S75 ＋ MT1）;（4） Nutrient solution ＋ 75 mmol L^-1 Na Cl ＋ spraying 50 μmol L^-1 MT（S75 ＋ MT2）;（5） Nutrient solution ＋ 75 mmol L^-1 Na Cl ＋ spraying 100 μmol L^-1 MT（S75 ＋ MT3）;（6） Nutrient solution ＋75 mmol L^-1 Na Cl ＋ spraying 200 μmol L^-1 MT（S75 ＋ MT4）;（7） Nutrient solution ＋75 mmol L^-1 Na Cl ＋ spraying 400 μmol L^-1 MT（S75 ＋ MT5）. Each treatment had six boxes as duplicate, and solutions in the boxes were replaced every other day during the whole culture

关 键 词：褪黑素 喷施 水稻幼苗 盐胁迫 氮磷钾营养 离子稳态 

分 类 号：S792.99[农业科学—林木遗传育种]