马蓝等79种植物分支酸合成酶的生物信息学分析  

Bioinformation analysis of chorismate synthase in Baphicacantus cusia and other 78 species of plants

作　　者：于剑 叶齐[1,2,3] 宁书菊 李卿[5] 谭何新[6] 冯静娴[5] 陈瑞兵 马晓莉[1,2,3] 公培民 赵璇璇 张磊 魏道智[1,2,3] YU Jian;YE Qi;NING Shu-ju;LI Qing;TAN He-xin;FENG Jing-xian;CHEN Rui-bing;MA Xiao-li;GONG Pei-min;ZHAO Xuan-xuan;ZHANG Lei;WEI Dao-zhi(College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fuzhou 350002, China;Key Laboratory of Crop Ecology and Molecular Physiology, Ftazhou 350002, China;College of Crop Science , Fujian Agriculture and Forestry University, Fuzhou 350002, China;Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China)

机构地区：[1]福建农林大学生命科学学院,福建福州350002 [2]福建省农业生态过程与安全监控中重点实验室,福建福州350002 [3]作物生态与分子生理学福建省高校重点实验室,福建福州350002 [4]福建农林大学作物科学学院,福建福州350002 [5]第二军医大学附属长征医院,上海200003 [6]第二军医大学药学院药用植物学教研室,上海200433

出　　处：《中国中药杂志》2018年第4期721-730,共10页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81573517,31670292);国家自然科学基金-促海峡两岸科技合作联合基金项目(U1405215)

摘　　要：分支酸合成酶（ehorismate synthase，CS，EC：4．2．3．5）催化5-烯醇式莽草酸-3-磷酸生成分支酸，是生物体内分支酸合成所必须的酶。目前，Genbank报道了79种高等植物cS的氨基酸序列。该研究采用生物信息学方法对马蓝等79种植物共125条cs氨基酸序列的组成成分、信号肽、导肽、疏水性／亲水性、跨膜结构、卷曲螺旋结构、蛋白二级结构、三级结构及其功能域等进行预测和分析，并构建了cs蛋白家族的系统进化树。进化分析结果表明这79种植物的cs被分为8个类群；而氨基酸序列同源性比对结果显示，马蓝cs的氨基酸序列与芝麻、烟草、马铃薯等植物的同源性比较高。所有植物cs的开放阅读框在1300bp左右，相对分子质量为50kDa左右，等电点（pI）在5．0-8．0，呈微碱性。该研究克隆得到的马蓝cs的开放阅读框为1326bp，氨基酸残基数为442个，相对分子质量47kDa，等电点（pI）为8．11。CS氨基酸肽链表现出明显的疏水区和亲水区，不存在信号肽，可能存在跨膜结构域。蛋白质二级结构中最主要的结构元件是无规则卷曲和d．螺旋，并含有活性结构域、PLN02754保守域和FMN结合位点3个主要的组成结构域。研究所获得的结构信息，可为今后进一步深入研究植物cs的构效关系和结构改造提供一定的科学依据。Chorismate synthase （CS, EC： 4. 2. 3.5） catalyses 5-enolpyruvy-shikimate-3-phosphate to form chorismate, which is the essential enzyme for chorismate biosynthesis in organisms. The amino acid sequences of CS from 79 species of higher plants were reported in GenBank at present. 125 amino acid sequences ofCS from Baphiacanthus cusia and other 78 species of plants were predic- ted and analyzed by using various bioinformatics software, including the composition of amino acid sequences, signal peptide, leader peptide, hydrophobic/hydrophilic, transmembrane structure, coiled-coil domain, protein secondary structure, tertiary structure and functional domains. The phylogenetic tree of CS protein family was constructed and divided into eight groups by phylogenetic analysis.The homology comparison indicated that B. cusia shared a high homology with several plants such as Sesamum indicurn, Nicotiana tabacum, Solanwm tuberosum and st） on. The open reading frame （ORF） of all samples is about 1 300 bp, the molecular weight is about 50 kDa, the isoelectric point （pI） is 5.0-8.0 which illustrated that CS protein is slightly basic. The ORF of CS we cloned in B. cusia is 1 326 bp, the amino acid residues are 442, the molecular weight is 47 kDa and pI is 8.11. The CS in B. cusia showed ob- vious hydrophobicity area and hydrophilicity area, no signal peptide, and may exists transmembrane structure areas. The main seconda- ry structures of CS protein are random coil and Alpha helix, also contain three main structural domains which are an active structural domain, a PLN02754 conserved domain and a FMN binding site. The acquired information in this study would provide certain scientific basis for further study on structure-activity relationship and structure modification of CS in plants in the future.

关 键 词：马蓝 分支酸合成酶 生物信息学 构效关系 结构改造 

分 类 号：S567.239[农业科学—中草药栽培]