芍药苷、薄荷脑对葛根素在Calu-3细胞模型转运过程中膜流动性、钠-钾离子ATP酶和钙离子ATP酶的影响  

Effect of paeoniflorin and menthol on membrane fluidity,Na+-K+-ATPase activity and Ca2+-ATPase activity during transport of puerarin in Calu-3 cell

作　　者：张林 王停 杜守颖[2] 陆洋[2] 范志恒 马军明[2] 谭佳威 薛宇涛 

机构地区：[1]北京中医药大学北京中医药研究院,北京100029 [2]北京中医药大学中药学院,北京100029

出　　处：《中国中药杂志》2018年第4期731-735,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(81473363)

摘　　要：研究芍药苷、薄荷脑对葛根素在细胞模型转运过程中对Calu-3细胞膜生理功能的影响。以Calu-3细胞作为模拟鼻黏膜组织的体外细胞模型，采用荧光漂白恢复技术及超微量酶活性检测方法，考察细胞膜流动性、Na＋-K’-ATP酶和ca^2＋．ATP酶活性，探索配伍药物促进葛根素转运的作用机制。结果表明，配伍低、中、高浓度薄荷脑或同时配伍低、中、高浓度芍药苷和薄荷脑时，与单独使用葛根素相比，Calu-3细胞膜荧光漂白恢复率显著升高，Na^＋-K＋-ATP酶活性未发生明显改变，Ca^2＋-ATP酶活性显著增强，由此证实薄荷脑起到主要渗透促进作用，并且其作用机制可能与增加流动性、激活Ca^2＋-ATP酶活性密切相关。The aim of this research is to investigate the effects of paeoniflorin and menthol on the physiological function of Calu-3 cell membrane during the transport of puerarin. Calu-3 cell was used as the in vitro cell model to simulate nasal mucosa tissues, and the cell membrane fluidity, Na＋ -K ＋ -ATPase activity and Ca2＋ -ATPase activity were detected by fluorescence recovery after photob- leaching （FRAP） and ultramicro enzyme, activity testing, in order to explore the mechanism of c0mpatible drugs on promoting puerarin transport. The results showed that ^-hen puerarin associated with low, middle and high concentration of menthol or both paeoniflorin and menthol, the fluorescence recovery rate was increased significantly, while Na＋ -K＋ -ATPase activity had no significant change and Ca2＋ -ATPase activity was enhanced significantly as compared with puerarin alone. Therefore, it was concluded that menthol had the ability of promoting the transport and the mechanism might bo related to increasing membrane fluidity and activating Ca2＋-ATPase.

关 键 词：薄荷脑 Calu-3细胞 细胞膜流动性 NA+-K+-ATP酶 CA2+-ATP酶 

分 类 号：R285[医药卫生—中药学]