红车轴草中nNOS-PSD-95解偶联剂的高效发现和快速筛选  

Efficient discovery and capturing of nNOS-PSD-95 uncouplers from Trifolium pratense

作　　者：何宏亮 潘林丽 顾小丽 黄姣姣[2] 孙成红 唐玉林 陈立娜[2] HE Hong-liang;PAN Lin-li;GU Xiao-li;HUANG Jiao-jiao;SUN Cheng-hong;TANG Yu-lin;CHEN Li-na(Department of Clinical Pharmacy, Sir Run Run Hospital, Nanjing Medical University, Nanjing 211166, China;School of Pharmacy, Nanfing Medical University, Nanfing 211166, China;Nantong First People＇s Hospital, Nantong 226001, China)

机构地区：[1]南京医科大学附属逸夫医院临床药学部,江苏南京211166 [2]南京医科大学药学院,江苏南京211166 [3]南通市第一人民医院,江苏南通226001

出　　处：《中国中药杂志》2018年第4期748-754,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30801558,81373895,81173538)

摘　　要：以ZL006为模板分子，丙烯酰胺（AA）为功能单体，乙腈为致孔剂，制备磁性分子印记聚合物（MMIPs），并且通过傅立叶红外光谱仪（FT-IR）和场发射扫描电子显微镜（sEM）对其进行形态和结构表征；将制备的磁性分子印记聚合物作为人工受体从红车轴草中直接分离提取新的nNOS-PSD-95解偶联剂，并且通过细胞神经保护作用和免疫共沉淀实验对所筛选化合物进行活性评价。结果表明，成功合成的MMIPs具有较好的分散性、适宜的粒径大小和良好的吸附性能，并从红车轴草中分离富集得到刺芒柄花素、樱黄素、鹰嘴豆芽素A，细胞神经保护作用和免疫共沉淀结果显示鹰嘴豆芽素A具有较好的细胞保护作用和解偶联活性。Magnetic molecularly imprinted polymers （MMIPs） were prepared with ZL006 as template, acrylamide （AA） as the functional monomer, and acetonitrile as pore-forming agent; then Fourier transform infrared spectroscopy （FT-IR） and scanning elec- tron microscopy （SEM） were used to characterize their forms and structures. Simultaneously, the MMIPs prepared previously were used as sorbents for dispersive magnetic solid phase extraction （DSPE） to capture and identify potential nNOS-PSD-95 uncouplers from extracts of Trifolium pratense and the the activities of the screened compounds were evaluated by the neuroprotective effect and co-im- munoprecipitation test. The experiment revealed that the successfully synthesized MMIPs showed good dispersiveness, suitable particle size and good adsorption properties. Formononetin, prunetin and biochanin A were separated and enriched from Trifolium pratense by using the MMIPs as artificial antibodies and finally biochanin A was found to have higher cytoprotective action and uncoupling action according to the neuroprotective effect and co-immunoprecipitation test.

关 键 词：红车轴草 nNOS-PSD-95解偶联剂 磁性分子印记聚合物 脑卒中 快速发现 ZL006 

分 类 号：R284[医药卫生—中药学]