甘肃不同产地及不同生长年限红芪和黄芪中4种异黄酮类成分的含量对比  

Comparison on Contents of 4 Kinds of Isoflavones in Hedysari Radix and Astragali Radix from Different Habitats and Different Years of Growth in Gansu

作　　者：叶迎[1] 王瑞海[1] 柏冬[1] 薛欣[1] 李玉梅[1] 张立石[1] 苗青[1] 许京[1] 刘丽梅[1] YE Ying;WANG Rui-hai;BAI Dong;XUE Xin;LI Yu-mei;ZHANG Li-shi;MIAO Qing;XU Jing;LIU Li-mei(Institute of Basic Theory Research of Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China)

机构地区：[1]中国中医科学院中医基础理论研究所

出　　处：《中国实验方剂学杂志》2018年第9期52-58,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院自主选题(YZ-1438)

摘　　要：目的：建立甘肃红芪和黄芪药材中4种异黄酮类成分含量测定的方法,比较甘肃不同产地一年生、二年生红芪和黄芪中4种异黄酮类成分含量。方法：以毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素为对照品,采用HPLC法,Agilent Eclipse-C18色谱柱（4.6 mm×250 mm,5μm）,乙腈-0.1%磷酸水梯度洗脱,在254 nm处检测。结果：毛蕊异黄酮苷在2.25-0.005 625μg（r=0.999 7）,芒柄花苷在2.22-0.111μg（r=0.999 4）,毛蕊异黄酮在0.112-0.001 12μg（r=0.999 9）,芒柄花素在1.68-0.001 68μg（r=0.999 8）与峰面积呈良好的线性关系。红芪和黄芪的平均加样回收率均在95%-105%,RSD均〈3%（n=6）。结论：该研究建立的红芪和黄芪中4种异黄酮类成分含量测定方法简便、准确、重复性好,可用于甘肃地区红芪和黄芪中4种异黄酮类成分含量测定及其对比研究。从4种异黄酮类成分含量分析,一年生、二年生红芪质量相当,一年生黄芪优于二年生,且黄芪质量优于红芪。红芪最佳产区为武都,黄芪最佳产区为宕昌。甘肃地区红芪芒柄花素含量高于黄芪,黄芪毛蕊异黄酮苷、毛蕊异黄酮、芒柄花苷含量高于红芪。在开发和利用甘肃红芪和黄芪时,应根据二者异黄酮含量各自优势有所选择,二者不适合替代使用。Objective：To establish a method for content determination of 4 kinds of isoflavones in Hedysari Radix and Astragali Radix in Gansu province,and compare the contents of 4 kinds of isoflavones from one year old and two years old samples from different areas of Gansu province.Method：Isoflavone glycosides,formononetin,myrion isoflavones and formononetin were used as the reference substance.Agilent Eclipse-C18 column（4.6 mm × 250 mm,5 μm） was used with acetonitrile-0.1% phosphoric acid solution as the mobile phase for gradient elution.The detection was conducted at the wavelength of 254 nm.Result：The isoflavone glycosides,formononetin,myrion isoflavones and ononin showed good linear relationship with the peak area at the range of 2.25-0.005 625 μg（r = 0.999 7）,2.22-0.111 μg（r = 0.999 4）,0.112-0.001 12 μg（r = 0.999 9）,and 1.68-0.001 68 μg（r = 0.999 8） respectively.The average recoveries of Hedysari Radix and Astragali Radix were 95%-105% with RSD 〈 3%（n = 6）.Conclusion：The method for the content determination of 4 kinds of isoflavones in Hedysari Radix and Astragali Radix was simple,accurate and reproducible.It can be used for the content determination of four isoflavones in Hedysari Radix and Astragali Radix from Gansu province and its comparative study.From the content analysis of 4 kinds of isoflavones,it was found that the quality of one year old Hedysari Radix was equivalent to that of two years old Hedysari Radix; one year old Astragali Radix had better quality than two years old Astragali Radix; and the quality of Astragali Radix was better than that of Hedysari Radix.The best producing area for Hedysari Radix was Wudu,and the best producing area for Astragali Radix was Tanchang.Formononetin content in Gansu Hedysari Radix was higher than that in Astragali Radix,while the contents of isoflavone glycosides,myrion isoflavones and ononin were lower than those in Astragali Radix.Therefore,the selection shall be made according to the isoflavones content during development and u

关 键 词：红芪 黄芪 异黄酮 高相液相色谱法 含量测定 甘肃 

分 类 号：R284.1[医药卫生—中药学] R22[医药卫生—中医学]