基于UPLC-Q-TOF/MS分析痰热清注射液在大鼠体内的入血成分  

Qualitative Analysis of Components Absorbed into Rat Blood After Administration of Tanreqing Injection by UPLC-Q-TOF/MS

作　　者：王志鹏[1] 孙亮[1,2,3] 张凤[1] 高守红[1] 陈万生[1] 柴逸峰[2] WANG Zhi-peng;SUN Liang;ZHANG Feng;GAO Shou-hong;CHEN Wan-sheng;CHAI Yi-feng(Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;School of Pharmacy, Second Military Medical University, Shanghai 200433, China;The 98th Hospital of Chinese People＇s Liberation Army, Huzhou 313000, China)

机构地区：[1]第二军医大学长征医院,上海200003 [2]第二军医大学药学院,上海200433 [3]中国人民解放军第98医院,浙江湖州313000

出　　处：《中国实验方剂学杂志》2018年第9期77-85,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家杰出青年科学基金项目(81325924);国家自然科学基金面上项目(81470173)

摘　　要：目的：采用UPLC-Q-TOF/MS对大鼠血液中痰热清注射液成分进行定性鉴别分析,为该制剂的临床应用提供参考。方法：采用Agilent Eclipse Plus C_（18）色谱柱进行色谱分离,流动相0.1%甲酸水溶液-乙腈梯度洗脱。在正、负离子模式下使用电喷雾离子源（ESI）模式获取数据,数据采集范围m/z 100-1 100,通过同对照品对比、质谱分析、检索文献等方法进行物质鉴别。结果：共鉴别分析出27种入血成分,获得其中5种主要指标成分绿原酸、黄芩苷、咖啡酸、熊去氧胆酸与鹅去氧胆酸的主要代谢产物及代谢途径。咖啡酸、绿原酸可生成多种Ⅰ相和Ⅱ相消除产物,代谢途径多样;而熊去氧胆酸、鹅去氧胆酸与黄芩苷则主要以Ⅱ相消除为主。结论：痰热清注射液中仅有部分次要成分不会被吸收入血。建立的UPLC-Q-TOF/MS可对痰热清注射剂入血成分进行快速有效的定性鉴别,并可确定主要成分的代谢状态。Objective：To qualitatively identify components of Tanreqing injection in rat blood based on UPLC-Q-TOF/MS.Method：The chromatographic separation was carried out on an Agilent Eclipse Plus C_（18） column,mobile phase was 0.1% formic acid in water and acetonitrile for gradient elution.Electrospray ionization（ESI） was adopted to obtain data under positive and negative mode,the data was collected in the range of m/z100-1 100,and components were identified by comparing with reference substances,analysing MS and retrieving literatures.Result：A total of 27 components from Tanreqing injection were identified in rat blood,main metabolites and metabolic pathways of five main components（caffeic acid,chlorogenic acid,baicalin,ursodesoxycholic acid and chenodeoxycholic acid） were obtained.Caffeic acid and chlorogenic acid could product a variety of elimination products of phase Ⅰ and Ⅱ with diverse metabolic pathways; baicalin,ursodesoxycholic acid and chenodeoxycholic acid were mainly elimination of phase Ⅱ.Conclusion：Only some of the secondary components in Tanreqing injection can not be absorbed into the blood.This UPLC-Q-TOF/MS can rapidly identify bioactive compounds of Tanreqing injection and the main metabolic status of them.

关 键 词：痰热清注射液 入血成分 代谢产物 咖啡酸 黄芩苷 绿原酸 熊去氧胆酸 

分 类 号：R24[医药卫生—中医临床基础] R289[医药卫生—中医学]