山杏体细胞胚胎发生及其组织学观察  

Somatic embryogenesis processes in Armeniaca sibirica and its histological observation

作　　者：何炎红[1] 吴高殷 白玉娥[1] 田春 HE Yanhong;WU Gaoyin;BAI Yu＇el;TIAN Chun(Forestry College, Inner Mongolia Agricultural University, Hohehot 010020, Inner Mongolia, China;Inner Mongolia Autonomous Region Hospital of Traditional Chinese Medicine, Hohehot 010010, Inner Mongolia, China)

机构地区：[1]内蒙古农业大学林学院,内蒙古呼和浩特010020 [2]内蒙古自治区中医医院,内蒙古呼和浩特010010

出　　处：《经济林研究》2018年第1期163-166,共4页Non-wood Forest Research

基　　金："十二.五"国家科技支撑项目"仁用杏和巴旦杏高效生产关键技术研究与示范"(2013BAD14B02)

摘　　要：为了建立山杏体细胞胚胎发生再生体系,以山杏茎尖为外植体,研究山杏体细胞胚胎发生及其主要影响因子。结果表明:山杏茎尖在MS+6-BA(0.5 mg/L)+NAA(0.2 mg/L)培养基中愈伤组织诱导率达100%,胚性愈伤组织增殖的最佳培养基为MS+6-BA(0.5 mg/L)+2,4-D(0.2 mg/L)+水解酪蛋白(0.5 g/L)。山杏体细胞胚胎发生过程中能明显看出球形胚、心形胚、鱼雷胚、子叶胚等阶段。Developmental process of somatic embryogenesis from stem tip in Armeniaca sibirica and main influence factors were studied,to establishment regeneration system of somatic embryogenesis for A.sibirica.The results show that callus induction rate of stem tip in A.sibirica was 100% in the medium of MS＋6-BA（0.5 mg/L） ＋ NAA（0.2 mg/L）,and the optimal medium for embryonic callus proliferation was MS＋6-BA（0.5 mg/L）＋2,4-D（0.2 mg/L） ＋ CH（0.5 g/L）.The processes of somatic embryogenesis in A.sibirica had obvious stages of global embryo,heart embryo,torpedo embryo and cotyledon embryo.

关 键 词：山杏 愈伤组织 体细胞胚胎 组织学观察 

分 类 号：S662.2[农业科学—果树学]