气相色谱-质谱法分析柚木光辐射前后的抽提物成分  

Analysis of extractive compounds in teakwood before and after irradiation by chromatography-mass spectrometry

作　　者：李慧 曾栋[2] 黄自知[1] 马强[1] 王琼[1] 胡云楚[1] 文瑞芝[1] LI Hui;ZENG Dong;HUANG Zizhi;MA Qiang;WANG Qiong;HU Yunchu;WEN Ruizhi(College of Science, Central South University of Forestry ＆ Technology, Changsha 410004, Hunan, China;Hunan Center of Disease Control and Prevention, Changsha 410005, Hunan, China)

机构地区：[1]中南林业科技大学理学院,湖南长沙410004 [2]湖南省疾病预防控制中心,湖南长沙410005

出　　处：《中南林业科技大学学报》2018年第2期122-128,共7页Journal of Central South University of Forestry & Technology

基　　金：国家自然科学基金项目(31670563);湖南省教育厅项目(14C1188)

摘　　要：采用气相色谱-质谱法分析柚木光辐射前后的抽提物成分,考察了柚木光辐射前后的颜色和组成变化,确定了可能引起柚木光变色的主要成分。结果显示,柚木光照100 h后黄蓝指数变化最为明显,表面变成金黄色,从柚木光辐射前后的抽提物中共鉴定出76种成分,其中柚木光辐射前的抽提物中鉴定出55种成分,柚木光辐射后的抽提物中鉴定出49种成分,包括烃类、醇类、酚类、酯类、醛类、酮类、蒽醌类和胺类,辐射后的柚木抽提物中烯烃类从20.11%减少至8.80%,由5种烯烃类减少为4种;酮类从0.99%增加到9.19%,由5种酮类增加为9种;杂环类化合物从20.56%减少到1.54%,由6种杂环类减少为1种。柚木单板表面光辐射后变亮、变黄,随着辐射时间加长,可变成受人欢迎的金黄色,这可能与抽提物中化合物成分的种类和含量有关,木材抽提物中的酚羟基、羰基、双键等结构与木材光变色有很大关系。柚木光辐射前抽提物的成分主要为反式角鲨烯(20.108%)、2-甲基蒽醌(13.110%)、1-甲基-3,4-二氢异喹啉(12.452%)、2-羟甲基蒽醌(8.210%)、8-甲氧基-1-甲基-.beta.-咔啉(6.552%)这5种化合物,这些主成分都含有与木材光变色相关的双键或者羰基。辐射后除了8-甲氧基-1-甲基-.beta.-咔啉和1-甲基-3,4-二氢异喹啉检测不到以外,相对百分含量减少较大的是反式角鲨烯(9.970%)和2-羟甲基蒽醌(5.368%),只有2-甲基蒽醌(13.678%)相对百分含量增加。而且由于2-甲基蒽醌为黄色晶体,因此推测柚木表面黄色的深浅可能与有效成分2-甲基蒽醌的量有关,但柚木光变色的具体原因和机理还有待进一步研究。Chromatography-mass spectrometry was used to analyze extractive compounds in Teakwood before and after irradiationandthe variations of the color and compositions in Teakwood before and after irradiation was studied.The results showed that the changes of yellow blue index was most significant and Teakwood surface can turn into golden yellow.Besides,there were 76 kinds of compounds were identified in all and 55 compounds in Teakwood before irradiation,while there were 49 compounds in Teakwood after irradiation,including hydrocarbons,alcohols,phenols,esters,aldehydes,ketone,anthraquinone and amine.After irradiation,the content of alkenes decreased to 8.80% from 20.11% and the species decreased to 4 from 5;the ketone increased to 9.19% from 0.99% with the species of 9 from 5;the Heterocyclics decreased to 1.54% from 20.56% with the species of 1 from 6.After irridiation,Teak veneer surface become bright and yellow,it can turn into a popular golden yellow with the radiation time.The phenomenon may be related to the kinds and contents of the extractive,of which phenolic hydroxyl,carbonyl,double bonds may has a great effects on wood light-induced discoloration.The results indicate that the major components in Teakwood before irradiation were squalene（20.108%）,2-methyl-anthracenedione（13.110%）,1-Methyl-3,4-dihydroisoquinoline（12.452%）,2-（Hydroxymethyl）anthraquinone（8.210%）,8-methoxy-1-methyl-.beta.-Carboline（6.552%）,and those major constituents both contained double bonds or carbonyl groups that related to wood discoloration After irradiation,the 8-methoxy-1-methyl-.beta.-Carboline and 1-Methyl-3,4-dihydroisoquinoline disappeared;the contents of squalene and 2-（Hydroxymethyl）anthraquinone decreased to 9.970% and 5.368%,respectively;and the content of 2-methylanthracenedione increased by 13.678%.Besides,2-methyl-anthracenedione was a yellow crystalline so that we concluded that 2-methylanthracenedione may contribute to increasing yellow of Teak veneer,but the light-induced discoloration reason a

关 键 词：柚木 抽提物 光辐射 光变色 气相色谱-质谱法 

分 类 号：S796[农业科学—林木遗传育种]