止泻散质量标准研究  

Study on the quality control of Zhixei Powder

作　　者：尚小飞 潘虎[1] 胡宏伟 王瑜 汪晓斌 张继瑜[1] 苗小楼[1] SHANG Xiaofei;PAN Hu;HU Hongwei;WANG Yu;WANG Xiaobin;ZHANG Jiyu;MIAO Xiaolou(Key Laboratory of New Animal Drug Project, Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Lanzh0u Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Science, Lanzhou Gansu 730050, China;Husbandry and Veterinary Medicine Institute of Lanzhou City, Gansu Province, Lanzhou Gansu 730050, China)

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验室甘肃省新兽药工程重点实验室,甘肃兰州730050 [2]甘肃省兰州市畜牧兽医研究所,甘肃兰州730050

出　　处：《中兽医医药杂志》2018年第2期66-69,共4页Journal of Traditional Chinese Veterinary Medicine

基　　金：兰州市人才创新创业项目(2014-RC-74)

摘　　要：为了控制止泻散的质量,采用高效液相色谱法测定制剂中槲皮素和盐酸小檗碱的含量。结果显示,色谱条件为甲醇-0.4%磷酸溶液（50∶50）、检测波长360 nm、柱温30℃时,止泻散制剂中槲皮素含量为0.054 mg/g;色谱条件为乙腈-水（47∶53）、检测波长345 nm、柱温30℃时,盐酸小檗碱含量为0.632 mg/g。该方法简便、灵敏、无干扰,可以有效控制止泻散的质量。In order to control the quality of Zhixie Powder,high performance liquid chromatography（HPLC）was used to determine the content of quercetin and berberine in the samples.The results showed that the optimized chromatographic condition for determining quercetin in Zhixie Powder was methanol（50%）and 0.4% phosphoric acid solution（50%）of the solvent system at 360 nm,and the content was 0.054 mg/g.The chromatographic condition for determining berberine was acetonitrile（47%）and water（53%）of the solvent system at 345 nm,and the content of berberine was 0.632 mg/g.The methods are easy and precise,and could be used to control the quality of Zhixie Powder.

关 键 词：止泻散 HPLC 槲皮素 小檗碱 

分 类 号：S853.7[农业科学—临床兽医学]