早期致密化对IVF-ET临床妊娠结局的影响  

Effect of early compaction on clinical pregnancy results in IVF-ET

作　　者：王建业[1] 刘海萍[1] 唐宁[1] WANG Jian-ye;LIU Hai-ping;TANG Ning(Reproductive Medicine Center of Jinan Military General Hospita)

机构地区：[1]济南军区总医院生殖医学中心,济南250031

出　　处：《中国优生与遗传杂志》2018年第4期94-97,F0003,共5页Chinese Journal of Birth Health & Heredity

摘　　要：目的研究培养第3天胚胎致密化对体外受精-胚胎移植(IVF-ET)后临床妊娠结局的影响,优化移植前胚胎优选条件,改善IVF-ET的临床妊娠结局。方法 1.将接受IVF的患者分成非致密化组(A组,150例)和致密化组(B组,150例),比较相对应的两组病人的基本情况和受精后胚胎的发育情况以及移植后的临床妊娠情况;2.将接受IVF的患者分组成非致密化组(A组,100例)和致密化组(B组,100例),比较相对应的两组胚胎在受精后第5天囊胚的发育情况和移植后的临床妊娠情况;3.将培养第3天冷冻复苏后的胚胎分成非致密化组(A组,100例)和致密化组(B组,100例),比较相对应的两组胚胎在培养后囊胚的发育情况和移植后的临床妊娠情况。结果 (1)两组患者的年龄、不孕年限、Gn用量、基础FSH、移植日内膜厚度、获卵数、移植胚胎数、受精率、卵裂率、D3优质胚胎率、D3可用胚胎率、D3可用胚胎率和冷冻胚胎率均无统计学差异(P>0.05),但B组的临床妊娠率、着床率和活产率均显著地高于A组(P=0.05,P=0.006和P=0.046);(2)两组患者的年龄、不孕年限、Gn用量、基础FSH、移植日内膜厚度、获卵数、移植胚胎数、受精率、卵裂率、D3优质胚胎率、D3可用胚胎率和可用囊胚率没有统计学差异(P>0.05),与A组相比,B组患者移植后的临床妊娠率、着床率和活产率有明显的增加(P=0.047,P=0.003和P=0.033);(3)两组患者在D3可用胚胎率、可用囊胚率、移植日内膜厚度和移植胚胎数上没有统计学差异(P>0.05),但B组患者的临床妊娠率、着床率和活产率明显高于A组,两者有统计学差异(P=0.045,P=0.006和P=0.047)。结论第3天发生致密化的胚胎有更好的胚胎发育潜能,移植致密化的胚胎能提高临床妊娠率,改善临床妊娠结局,致密化的胚胎可以作为IVF-ET中的优选胚胎。Objective：To investigate the effect of compaction of embryos cultured in day 3 on clinical pregnancy results after transplantation,to optimize the selection of pre-transplanted embryo and to improve the clinical pregnancy result of IVF-ET. Methods：1,The patients undertaking IVF were divided into non-compacting group（Group A,150 cases）and compacting group（Group B,150 cases） and then the basic information,embryo development after fertilization and clinical pregnancy results after transplantation were comparatively studied;2,The patients undertaking IVF were divided into non-compacting group（Group A,100 cases）and compacting group（Group B,100 cases） and then the basic information,blastocyst development after fertilization and clinical pregnancy results after blastocyst transplantation were comparatively analyzed;3,Day 3 freezing-thawed embryos were divided into non-compacting group（Group A,100 cases）and compacting group（Group B,100 cases）and then development of blastocysts and clinical pregnancy results after blastocyst transplantation between the two groups were comparatively investigated. Results：（1） The age,duration of infertility,dosage of Gn used,basic FSH,thickness of endometrium,number of oocytes retrieved,number of embryo transplanted,fertilization rate,cleavage rate,Day 3 good quality embryo rate,Day 3 available embryo rate and cryopreservation rate between the two groups had no statistical difference（P〉0.05）,while clinical pregnancy rate,implantation rate and delivery rate in group B were significantly higher than those in group A（P=0.050,P=0.006 and P=0.046,respectively）;（2） The age,duration of infertility,dosage of Gn used,basic FSH,thickness of endometrium,number of oocytes retrieved,number of embryo transplanted,fertilization rate,cleavage rate,Day 3 good quality embryo rate,Day 3 available embryo rate and available blastocyst rate between the two groups were not statistically different（P〉0.05）,but clinical pregnancy rate,implantation rate and delive

关 键 词：体外受精 胚胎致密化 胚胎发育 胚胎移植 临床妊娠 

分 类 号：R321.33[医药卫生—人体解剖和组织胚胎学]