土壤反硝化培养气体中N2和O2的气相色谱测定方法  

Determination of N_2 and O_2 in Cultivated Gas of Denitrificated Soil by Gas Chromatography

作　　者：许丽卫[1] 王玲[1] 陈闻 耿梅梅[1] 张丽萍[1] 袁红朝[1] 李春勇[1] 彭灿 王久荣[1] XU Li-wei;WANG Ling;CHEN Wen;GENG Mei-mei;ZHANG Li-ping;YUAN Hong-zhao;LI Chun-yong;PENG Can;WANG Jiu-rong(Key Laboratory ＇for Agroecological Processes in Subtropical Region ,Research Institute of Subtropical Agriculture, The Chinese Academy of Sciences ,Changsha 410125)

机构地区：[1]中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,湖南长沙410125

出　　处：《分析科学学报》2018年第2期253-256,共4页Journal of Analytical Science

基　　金：中国科学院仪器功能开发专项基金(No.2015gw05)

摘　　要：为测定土壤反硝化培养气体中的N_2和O_2的浓度,利用厌氧箱制备样品,建立了自动进样器阀进样、填充柱分离,气相色谱-热导检测器测定的方法。N_2和O_2在2min内达到基线分离。对于低浓度的样品(N2≤1.0%,O2≤0.254%),使用厌氧箱后N_2和O_2测定的峰面积比未使用时相比降低约20.0%。目标分析物的保留时间和峰面积的相对标准偏差(RSD)分别小于0.1%和1.0%。N_2和O_2的线性范围分别为780~60 100μmol/mol和290~20 000μmol/mol,线性相关系数均在0.999以上,检出限分别为232.7μmol/mol和87.5μmol/mol。厌氧箱的使用很大程度上避免了空气中的N_2和O_2进入,使培养气体中低含量N_2和O_2的测定更加准确。An integrated gas chromatographic system has been developed and implemented for measurement of nitrogen and oxygen in the gas of denitrificated soil cultivation. The system employed a practical, effective configuration consisting of the sample preparation in anaerobic tank, auto-injection by valve, separation by packed column and one six-port valve in series with a TCD detector for detection. Separation of two target analytes was achieved in one single analysis within 2 rain. The peak area of the sample（N2 ≤1.0%, 02 ≤0. 254%） was reduced by 20% in anaerobic chamber. The reproducibility of retention times and peak area for N2 and 02 were found to be less than 0. 1% and 1.0%, respectively. There were linear relationships between peak areas and cencentrations in the range of 780-60 100 μmol/mol for N2 and 290-20 000μmol/mol for 02 ,respectively;with detection limits of 232.7 μmol/mol for N2 and 87.5 μmol/mol for 02. The use of anaerobic chamber largely avoided the enterance of nitrogen and oxygen from the air into cultivation gas,making the determination results more accurate.

关 键 词：土壤反硝化 厌氧箱 气相色谱法 N2 O2 

分 类 号：O657.71[理学—分析化学]