瑞利光散射技术快速测定鲜苹果中的苹果酸  

Fast Determination of Malic Acid in Fresh Apple by Rayleigh Light Scattering Technique

作　　者：曾晓莎 庞向东[1] 江虹[1] ZENG Xiao-sha;PANG Xiang-dong;JIANG Hong.(College of Chemistry and Chemical Engineering ,Collaborative Innovation Center of Green Development in Wuling Mountain Areas ,Yangtze Normal University ,Chongqing 408100)

机构地区：[1]长江师范学院化学化工学院,武陵山片区绿色发展协同创新中心,重庆408100

出　　处：《分析科学学报》2018年第2期287-290,共4页Journal of Analytical Science

基　　金：重庆市教委科技基金(No.KJl401226);长江师范学院科技基金(No：2015CXX080)

摘　　要：在碱性Tris-HCl缓冲介质中,苹果酸与维多利亚蓝B反应生成蓝色二元离子缔合物,使瑞利光散射明显增强,在368nm波长处,瑞利散射增强程度(ΔIRLS)与苹果酸的质量浓度在0.002~0.27mg/L范围内呈线性关系,检出限为0.0018mg/L。实验探讨了适宜的反应条件及主要分析化学性质。该方法简便、快速,用于市售鲜苹果中苹果酸含量的测定,回收率为98.4%~101%,相对标准偏差(RSD,n=5)为1.8%~2.2%。In the alkaline Tris-HC1 buffer medium,malic acid reacts with vitoria blue B to form a blue binary ionic association complex, which led to a distinctly enhanced Rayleigh light scattering（RLS）. At the wavelengthof 368 nm, the intensity of Rayleigh light scattering enhancement（AIaLs） was directly proportional to the mass concentration of malic acid in the range of 0. 002-0.27 mg/L with the detection limit of 0. 0018 mg/L. The method is simple,rapid,and used for the determination of malic acid content in fresh apple. The recovery and relative standard deviations （RSDs,n=5） were in the ranges of 98.4% -101% and 1.8%-2.2% ,respectively.

关 键 词：苹果酸 维多利亚蓝B 瑞利光散射 

分 类 号：O657.39[理学—分析化学]