模拟100m氦氮氧常规潜水对家兔氧化应激和炎性反应的影响  

Effects of simulated 100 m Trimix conventional diving on oxidative stress and inflammatory reaction in rabbits

作　　者：李洋洋[1] 石路 张延猛[2] 肖婵娟 刘洪涛[1] LI Yang-yang;SHI Lu;ZHANG Yan-meng;XIAO Chan-juan;LIU Hong-tao(Shanghai Jiao Tong University Underwater Engineering Insititute Co. Ltd;Shanghai Jiao Tong University, Institute of Underwater Technology;Shanghai Jiao Tong University-Chiba University International Cooperative Research Center, Shanghai 200231, China)

机构地区：[1]上海交通大学海洋水下工程科学研究院有限公司 [2]上海交通大学海洋水下工程科学研究院 [3]上海交通大学-千叶大学国际合作研究中心,上海200231

出　　处：《中国应用生理学杂志》2018年第1期1-3,7,共4页Chinese Journal of Applied Physiology

基　　金：中华人民共和国科学技术部专项基金(2012EG124043)

摘　　要：目的:探讨模拟100 m氦氮氧(Trimix)常规潜水对家兔氧化应激和炎性反应的影响。方法:空白组家兔8只,模拟100 m Trimix;常规潜水组家兔8只。模拟潜水按照Haldane理论计算所得的水下阶段减压表减压。Elisa法检测家兔模拟潜水前、后血清超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、过氧化氢酶(CAT)、丙二醛(MDA)和过氧化脂质(LPO)等氧化/抗氧化指标的变化。同样采用Elisa法检测空白组和潜水组家兔肺、脑组织γ干扰素(IFN-γ)、肿瘤坏死因子-α(TNF-α)、白介素6(IL-6)、白介素8(IL-8)、髓过氧化物酶活力(MPO)和基质金属蛋白酶9(MMP-9)等炎性因子的表达,并比较组织湿干比(Wet/Dry ratio)。结果:家兔潜水后SOD和GSH活性明显降低(P<0.01),CAT、MDA和LPO含量明显升高(P<0.01);潜水组家兔肺、脑组织中IL-8、IL-6、IFN-γ、TNF-α、MMP-9和MPO的表达均较空白组显著升高(P<0.05,P<0.01);潜水组家兔肺、脑组织湿干比与空白组差异不显著。结论:模拟100 m Trimix常规潜水对家兔氧化应激和炎性反应有显著影响。Objective： To investigate the effects of simulated 100 m Trimix conventional diving on tissue inflammatory cytokines in rabbits. Methods： Eight New Zealand rabbits were performed a simulated 100 m Trimix conventional diving program which was established according to the Haldane theory. The expression levels of interferon-gamma（ IFN-γ）,tumor necrosis factor-α（ TNF-α）,interleukin-6（ IL-6）,interleukin-8（ IL-8）,myeloperoxidase（ MPO） and matrix metallo proteinase-9（ MMP-9） in rabbits lung and brain tissues were detected by Elisa after diving decompression. The tissue wet/dry ratio was calculated. The serum levels of superoxide dismutase（ SOD）,glutathione（ GSH）,catalase（ CAT）,malondiadehyde（ MDA） and lipid peroxide（ LPO） were detected by Elisa method in rabbits before and after diving. Results： The expressions of IFN-γ,TNF-α,IL-6,IL-8,MPO and MMP-9 in simulated diving group rabbits were significantly increased compared with the intact group（ P〈0.05,P〈 0.01）; the simulated diving rabbits tissues wet/dry ratio had no significant changes compared with the intact group. After diving,the activities of SOD and GSH were decreased significantly（ P〈0.01）,while the contents of CAT,MDA and LPO were increased significantly（ P〈0.01）. Conclusion： The simulated 100 m Trimix conventional diving had significant impact on oxidative stress and inflammatory reaction in rabbits,the results of wet/dry ratio showed that the diving rabbits had no tissue edema after decompression.

关 键 词：氦氮氧常规潜水 炎性反应 氧化应激 湿干比 

分 类 号：R84[医药卫生—航空、航天与航海医学]