黄芪总黄酮对病毒性心肌炎小鼠心律失常与内质网应激因子的影响  

Effects of total flavonids of astragalus on arrhythmia,endoplasmic reticulum stress in mice with viral myocarditis

作　　者：刘灏[1] 呼日乐巴特[1] 熊英 魏成喜[3] 宣丽颖[3] 王羽[3] 赵明[1] LIU Hao;HURILE Bate;XIONG Ying;WEI Cheng-xi;XUAN Li-ying;WANG Yu;ZHAO Ming(Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao 028000;the First Clinical College of Inner Mongolia Medical University, Hohhot 100059;Medical College of Inner Mongolia University for Nationalities, Tongliao 028000, China)

机构地区：[1]内蒙古民族大学附属医院,通辽028000 [2]内蒙古医科大学第一临床学院,呼和浩特100059 [3]内蒙古民族大学医学院,通辽028000

出　　处：《中国应用生理学杂志》2018年第1期16-18,22,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金支持项目(81360587)

摘　　要：目的:研究黄芪总黄酮(TFA)对病毒性心肌炎小鼠心律失常与内质网应激及缝隙连接蛋白作用,明确TFA抗病毒性心肌炎合并心律失常作用机制。方法:36只雄性Balb/c小鼠分为正常对照组、病毒性心肌炎组和TFA组(n=12),病毒性心肌炎组腹腔内无菌注射含0.1 ml/d 10-950 TCID柯萨奇B3病毒(CVB3),注射3 d制备Balb/c小鼠病毒性心肌炎模型,TFA组给予CVB3同时尾静脉注射0.1 ml TFA(20 mg/L),共7 d。实验结束后心电图检测心律失常发生率后处死小鼠,取心脏行HE染色,观察心肌病理改变,Western blot检测各组小鼠心肌细胞葡萄糖调节蛋白78(GRP78)、内质网应激信号通路因子激活转灵因子4(ATF4)及缝隙连接蛋白(Cx43)表达。结果:与正常组比较,病毒性心肌炎组GRP78与ATF4的表达显著升高(P<0.01),Cx43表达明显下降(P<0.01);与病毒性心肌炎组比较,TFA组小鼠心肌细胞GRP78与内质网应激信号通路因子ATF4表达明显减少(P<0.01),Cx43表达明显增多(P<0.01)。结论:TFA抗心律失常作用可能与缓解内质网应激及增加Cx43表达有关。Objective： To investigate the effects of total flavonids of astragalus（ TFA） on arrhythmia,endoplasmic reticulum stress and connexcin in mice with viral myocarditis and to clarify the mechanisms of TFA against viral myocarditis complicated with arrhythmia. Methods： Thirty-six male Balb/c mice were randomly divided into control group,viral myocarditis group and total flavonoids group（ n = 12）. The mice of viral myocarditis were intraperitonealy injected with 0.1 ml/day 10-950 TCID CVB3 for 3 days. The mice of TFA group were intraperitoneal injected with 0.1 ml/day 10-950 TCID CVB3 for 3 days and treated with 0.1 ml,20 mg/L TFA by tail vein injection. At the end of the experiment,arrhythmia was detected by electrocardiogram,the heart of mice were stained by HE,the expressions of glucose-regulated protein78（ GRP78）,endoplasmic reticulum stress signaling pathway factor activating transcription factor 4（ ATF4） and connexcin 43（ Cx43） were detected by Western blot. Results： The expressions of GRP78 and ATF4 were increased and the expression of Cx43 was decreased in viral myocarditis,while TFA inhibited these effect of viral myocarditis in heart of mice. Conclusion： The antiarrhythmic effect of TFA may be related to the alleviation of endoplasmic reticulum stress and the increase of Cx43 expression.

关 键 词：病毒性心肌炎 心律失常 内质网应激 缝隙连接蛋白 小鼠 

分 类 号：R331.3[医药卫生—人体生理学] R332[医药卫生—基础医学]