近端胃电刺激对大鼠摄食行为和胃肠功能的影响  

Effects of proximal gastric electrical stimulation on feeding behavior and gastrointestinal function in rats

作　　者：张超[1] 李非[1] 刘爽[2] 孙海晨[2] 高翔[1] 刘殿刚[1] 李昂[1] 李嘉[1] 方育[1] 张国君[3] Zhang Chao;Li Fei;Liu Shuang;Sun Haichen;Gao Xiang;Liu Diangang;Li Ang;Li Jia;Fang Yu;Zhang Guojun(Department of General Surgery, Xuanwu Hospital of Capital Medical University, Belling 100053, China)

机构地区：[1]首都医科大学宣武医院普通外科,北京100053 [2]首都医科大学宣武医院外科实验室,北京100053 [3]首都医科大学宣武医院功能神经外科,北京100053

出　　处：《中华普通外科杂志》2018年第4期330-333,共4页Chinese Journal of General Surgery

摘　　要：目的观察近端胃电刺激对sD大鼠体质量和胃肠运动的影响，探讨胃电刺激与血清胃肠激素的神经．体液调控的关系。方法12只sD大鼠分为实验组（6只）和对照组（6只），均置入胃电刺激器，实验组进行双通道串脉冲胃电刺激，连续观察刺激启动后4周内大鼠体质量、摄食水、排便的变化情况，在第4周通过建立小肠造瘘监测静息胃容积和胃排空情况，并检测血清胃肠激素。结果在胃电刺激启动后4周，实验组大鼠体质量、摄食和排便情况较对照组减少，差异均有统计学意义（体质量：t=4．005；摄食情况：t=2．530；排便情况：t=3．350，均P〈0．05）。胃电刺激4周后，实验组大鼠静息胃容积小于对照组[（2．93±0．50）ml比（5．10±0．53）ml，Z=2．460，P=0．014]，实验组大鼠30min内小肠造瘘引流量少于对照组，差异有统计学意义[（0．18±0．15）ml比（0．44±0．05）ml，Z=2．513．P=0．012]。两组大鼠血清胰高血糖素样肽1水平相比差异无统计学意义[（0．44±0．05）ml比（0．18±0．15）ml，Z=1．026．P=0．3051。实验组大鼠胃饥饿素水平高于对照组[（1．65±0．58）比（0．65±0．36），Z=2．380．P=0．017]。结论近端胃电刺激可导致大鼠体质量、摄食和排便功能改变,其可能通过刺激神经反射改变胃肠激素的水平及大鼠的胃肠动力和容积进而影响胃肠功能。Objective To observe the effects of proximal gastric electrical stimulation （GES） on body weight and gastrointestinal motility in SD rats, and investigate the regulation of gastric nerve stimulation and serum gastrointestinal hormones by neuro-humoral regulation. Methods 12 SD rats were divided into experimental group （n = 6） and control group （n = 6 ）, with gastric electrical stimulator implanted, and in experimental group dual-channel GES was activated. General status was observed for 4 weeks after GES activation, including body weight, feeding and water intake, urine and stool volumes, the resting gastric volume and gastric emptying were monitored via the establishment of intestinal fistula, and serum gastrointestinal hormones change was detected. Results During 4-week GES process, one rat had gastric retention and died at 1 week after GES activated. Compared with the control group, body weight, food intake, urine and stool volumes levels of the rats in experimental group decreased significantly （ t = 4. 005, 2. 530,3. 350, all P 〈 0. 05 ）. Resting gastric volume was significantly lower than that in the control group [ （2. 93± 0. 50） ml vs. （5.10 ±0. 53 ） ml, Z = 2. 460, P = 0. 014 ] , and the intestinal juice drainage was lower than the control group [ （ 0. 18 ± 0. 15 ） ml vs. （ 0. 44 ± 0. 05 ） ml, Z = 2. 513, P = 0. 012 ] , while serum GLP-1 levels were similar between the two groups [ （0. 44 ±0. 05）ml vs. （0. 18 ±0. 15）ml,Z = 1. 026, P = 0. 3051 , but Ghrelin was significantly higher than that in the control group [ （ 1.65±0. 58 ） vs. （0. 65 ± 0. 36）,Z = 2. 380, P = 0. 017 ]. Conclusion The proximal GES may lead to the change of the body weight, food intake, gastrointestinal function and motility, possibly by stimulating nerve reflex inducing gastrointestinal hormones secretion and affect gastrointestinal function.

关 键 词：电刺激 胃肠激素类 胃排空 

分 类 号：R656[医药卫生—外科学]