金黄色葡萄球菌噬菌体扩增方法的建立  

Development of a method for amplification of Staphylococcus aureus phage

作　　者：谢安平 魏东[2] 陈成[2] 董巧香[1] XIE An-ping;WEI Dong;CHEN Cheng;DONG Qiao-xiang(School of Laboratorial Medicine and Life Science, Wenzhou Medical University, Wenzhou 325035, Zhejiang Province, Chin)

机构地区：[1]温州医科大学检验医学院生命科学学院,浙江温州325035 [2]中国食品药品检定研究院结核病疫苗室,北京100050

出　　处：《中国生物制品学杂志》2018年第4期409-412,共4页Chinese Journal of Biologicals

摘　　要：目的建立金黄色葡萄球菌噬菌体扩增方法。方法通过比较培养体系中宿主菌浓度、噬菌体效价、培养时间及氯化钙对金黄色葡萄球菌噬菌体扩增的影响,确定最佳噬菌体扩增条件。结果经优化后,扩增培养体系中金黄色葡萄球菌宿主菌浓度为1×10~7个/mL,噬菌体效价为1×10~3 PFU/mL,扩增培养6~8 h,噬菌体效价达较高水平,氯化钙对噬菌体扩增无影响。结论建立了金黄色葡萄球菌噬菌体扩增方法,为金黄色葡萄球菌噬菌体治疗制剂的研制奠定了基础。Objective To develop a method for amplification of Staphylococcus aureus phage.Methods The condition for amplification was optimized by investigating the effect of host bacterial concentration,phage titer,culture time and the existence of calcium chloride on amplification of S.aureus phage.Results The optimal concentration of S.aureus in amplification cultivation system was 1 × 10-7 bacteria/mL,while the optimal phage titer was 1 × 10-3 PFU/mL,and the optimal culture time was 6-8 h.The existence of calcium chloride showed no effect on phage amplification,and the phage titer reached a relatively high level.Conclusion A method for amplification of S.aureus phage was developed,which laid a foundation of preparation of therapeutic S.aureus phage.

关 键 词：金黄色葡萄球菌 噬菌体 扩增 

分 类 号：Q939.99[生物学—微生物学]