陆地棉ADH基因家族的全基因组鉴定及表达分析  

Genome-wide Identification and Expression Analysis of the ADH Gene Family in Upland Cotton(Gossypium hirsutum L.)

作　　者：李常凤[1] 徐道青[1] 韩文兵[1] 张小娟[1] 齐苗[1] 添长久[1] 郑曙峰[1] Li Changfeng;Xu Daoqing;Han Wenbing;Zhang Xiaojuan;Qi Miao;Tian Changjiu;Zheng Shufeng(Cotton Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031)

机构地区：[1]安徽省农业科学院棉花研究所,合肥230031

出　　处：《分子植物育种》2018年第7期2065-2077,共13页Molecular Plant Breeding

基　　金：安徽省农业科学院院长青年创新基金项目(15B0722);安徽省农业科学院院长青年创新基金项目(17B0713);安徽省人才发展专项资金项目(17F0711);转基因生物新品种培育重大专项(2016ZX08005001-009)共同资助

摘　　要：本研究利用生物信息学方法对陆地棉ADH家族基因进行全基因组挖掘和分析,系统地解析Gh ADHs的序列特征、基因结构、染色体分布、非生物胁迫下基因表达模式以及蛋白进化关系、结构域特征与亚细胞定位等。结果表明:陆地棉基因组中共鉴定得到28个ADH,分布在16条染色体上;对应氨基酸可分为ADH1、FDH1、ADH0和BAD1等4个亚家族,长度从292~440个不等。均含有两个主要的结构域:催化结构域及辅酶结合结构域,且具有相同的亚细胞定位;表达分析显示大部分Gh ADHs对ABA、干旱、盐、碱、淹水、低温和高温等1种或多种胁迫表现出显著应答,暗示Gh ADHs在抵抗非生物胁迫中可能发挥重要作用。陆地棉ADH家族基因的鉴定与表达分析,为深入解析ADH响应棉花非生物胁迫中的功能及分子机制奠定了理论基础,进一步为棉花抗逆育种提供更多基因资源。In this research, we carried out genome-wide identification and analysis of ADH gene family in upland cotton （Gossypium hirsutum） by the bioinformatic method, and systematically analyzed sequence properties, gene structure, chromosome distribution and the expression pattern of GhA DHs under various abiotic stresses, as well as evolutionary relationships, conserved domains, subcellular localization of proteins. The results showed that twenty-eight putative GhA DHs were indentified, which were distributed on 16 chromosomes. The corresponding amino acids could be classified into ADH1, FDH1, ADH0 and BAD1 subfamilies, with the length varying from 292 to 440. They all had two major domains： catalytic domain and coenzyme binding domain, and possessed consistent subcellular localization. Analysis of expression profiles indicated that most GhADHs significantly responded to one or multiple stresses, such as ABA, drought, salt, alkali, waterlogging, cold and high temperature, suggesting that GhADHs played important roles in resisting abiotic stresses. The identification and expression analysis of ADH gene family in upland cotton laid a theoretical foundation for further analysis of the function and molecular mechanism of ADH response to abiotic stresses in cotton, and further provided more genetic resourcesfor cotton resistance breeding.

关 键 词：陆地棉 ADH基因家族 表达分析 非生物胁迫 

分 类 号：Q943.2[生物学—植物学] S562[农业科学—作物学]