甘蔗印度种响应干旱胁迫的数字基因表达谱  

Digital Gene Expression Profiling of S. barberi J. Response to Drought Stress

作　　者：李穆[1,2] 程志远[1,3] 何丽莲[1] 王先宏[1] 李富生[1] Li Mu;Cheng Zhiyuan;He Lilian;Wang Xianhong;Li Fusheng(College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201;Guangxi Institute of Subtropical Crops, Nanning, 530001;Institute of Crop Sciences ofCCAS, Beijing, 100081)

机构地区：[1]云南农业大学农学与生物技术学院,昆明650201 [2]广西壮族自治区亚热带作物研究所,南宁530001 [3]中国农业科学院作物科学研究所,北京100081

出　　处：《分子植物育种》2018年第7期2099-2106,共8页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31560417);云南省应用基础研究重点项目(2015FA024);云南省现代农业甘蔗产业技术体系建设专项(2009-2017)共同资助

摘　　要：本研究以甘蔗印度种的代表品种"春尼"为试材,采用数字基因表达谱(DGE)技术对干旱胁迫后的"春尼"进行差异基因表达分析,共筛选到差异表达基因9 810个,其中上调表达4 687个,下调表达5 123个。GO功能显著性富集分析表明:差异表达基因主要定位于细胞质、质体、叶绿体、类囊体等,主要行使催化活性、氧化还原酶活性、辅因子结合、裂解酶活性等功能,主要涉及小分子代谢、细胞酮体代谢、有机酸代谢、含氧酸代谢、化学刺激应答、非生物刺激应答等生物过程。KEGG显著性富集分析表明:差异表达基因主要涉及植物激素信号转导、RNA转运、次生代谢产物的生物合成、淀粉和蔗糖代谢、泛素化蛋白降解、嘌呤碱代谢等通路。利用实时荧光定量PCR(q RT-PCR)分析2个差异表达上调基因在"春尼"的苗期、伸长期、成熟期受干旱诱导的表达情况,结果均为"上调-上调-上调"模式,验证了DGE测序结果可靠、重复性好。In this research, we used Chunnee, the representative variety of S. barberi, as the test materials, and analyzed the differential gene expression of Chunnee under drought stress by digital gene expression profiling （DGE）. We totally screened out 9 810 differentially expressed genes which contained 4 687 up-regulated genes and 5 123 down-regulated genes. Go significant enrichment analysis showed that the differentially expressed genes mainly located in cytoplasm, plastid, chloroplasts, and envelope, which mainly had catalytic activity, oxidoreductase activity, cofactor binding, and lyase activity these functions. The biological processes mainly involved small molecule metabolism, cell ketone metabolism, organic acid metabolism, oxygen-containing acid metabolism, chemical stimulation response, non-biological stimulation response. KEGG significant enrichment analysis indicated that the differentially expressed genes mainly involved in pathways of plant hormone signal transduction, RNA transport, biosynthesis of secondary metabolites, starch and sucrose metabolism, ubiquitin mediated proteolysis, purine metabolism and so on. We used real-time fluorescent quantitative PCR （qRT-PCR） to analyze two differentially up-regulated expressed gene＇s expression patterns in seedling, elongation, maturity stages of Chunnee under drought stress, and the results showed the expression pattern was ＂up-regulated-up-regulated-up-regulated＂, which confirmed the DGE sequencing results were reliable and repeatable.

关 键 词：甘蔗印度种 春尼 干旱 数字基因表达谱 差异表达基因 

分 类 号：S423[农业科学—植物保护] S566.1